5S Ribosomal RNA gene repeat sequences define et least eight groups of plant trypanosomatids (Phytomonas spp.) : Phloem-restricted pathogens form a distinct section

Dollet Michel, Sturm Nancy R., Sanchez Moreno Manuel, Campbell David A.. 2000. 5S Ribosomal RNA gene repeat sequences define et least eight groups of plant trypanosomatids (Phytomonas spp.) : Phloem-restricted pathogens form a distinct section. Journal of Eukaryotic Microbiology, 47 (6) : 569-574.

Résumé : Trypanosomatids isolated from plants have been assigned typically into the genus Phytomonas. Such designations do not reflect the biology of the diverse isolates; confusion may arise due to the transient presence in plants of monogenetic (insect) trypanosomatids deposited by phytophagous bugs. To develop further molecular markers for the plant kinetoplastids, we have obtained the DNA sequence of the 5S ribosomal RNA gene from 24 isolates harvested from phloem, latex, and fruit. Small, distinct sequence differences were found at the 3'-ends of the transcribed regions; substantial sequence and size differences were found in the nontranscribed regions. Alignment of the gene sequences from all the isolates suggested the presence of eight groupings. While six groups contained isolates from single plant tissues, groups C and A contained isolates from both fruit and latex. The DNA sequences of the 10 phloem-restricted pathogenic isolates from South America and the Carribean were highly conserved and thus comprised a single group (H). The conserved nature of the 5S ribosomal RNA genes in these plant pathogens supports the proposal that they be considered as a distinct section, the phloemicola.

Mots-clés Agrovoc : Cocos nucifera, variation génétique, Trypanosoma, Protozoa, Mastigophora, PCR, huile de palme

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Dollet Michel, CIRAD-EMVT-SANTE ANIMALE (FRA)
• Sturm Nancy R., UC (USA)
• Sanchez Moreno Manuel, Universidad de Granada (ESP)
• Campbell David A., UC (USA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/476617/)

Métriques

[ Page générée et mise en cache le 2024-12-18 ]