Lorenzon Sophie, David Armelle, Nadew Melesse, Wesonga Hezron, Thiaucourt François. 2000. Specific PCR identification of the T1 vaccine strains for contagious bovine pleuropneumonia. Molecular and Cellular Probes, 14 (4) : 205-210.
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Résumé : A specific PCR test for the identification of the vaccine strains T1, T1/44 and T1 sr, was developed. This PCR reaction is based on variations of DNA sequences in a region flanking one IS1296 copy. The specific primer pair MmmSCP1-T1M2 amplifies a 700-bp long DNA fragment in the T1 vaccine strains and gives no amplification with the 60 other Mycoplasma mycoides subsp. mycoides SC strains tested. This PCR will permit to distinguish the T1 strain from all other vaccine strains and therefore avoid possible confusions. In addition, it should enable better investigations of post-vaccinal reactions.
Mots-clés Agrovoc : péripneumonie contagieuse bovine, Mycoplasma mycoides, vaccin, PCR
Classification Agris : L73 - Maladies des animaux
Auteurs et affiliations
- Lorenzon Sophie, CIRAD-EMVT-SANTE ANIMALE (FRA)
- David Armelle, CIRAD-EMVT-SANTE ANIMALE (FRA)
- Nadew Melesse, NVI (ETH)
- Wesonga Hezron, KARI (KEN)
- Thiaucourt François, CIRAD-EMVT-SANTE ANIMALE (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/476624/)
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