Podlipaev Sergei A., Frolov A., Bulat S., Banuls A.L., Dollet Michel.
1998. The diversity of insect and plant trypanosomatids: molecular (PCR) and morphology data.
In : The flagellates : International Symposium of the Systematics Association, Birmingham, September 7th-10th, 1998
Note générale : Résumé seul
Résumé : The most unreliable part in the taxonomic system of the trypanosomatids is the domain of trypanosomatids from insects and plants. The relationships within insect trypanosomatids are still obscure. Twenty-two isolates from insects (both reference strains and recent isolates) and four isolates from plants were, investigated by RAPD, UP-PCR and cross dot-blot hybridization of UP-PCR products. Ultrastructure and some additional characters (polymorphism of colonies on solid medium, DNA content by flow cytometry etc.) were also examined. It was concluded that: I. The main clusters on dendrograms derived from PCR are supported by ultrastructural data; 2. Leptomonas-like isolates are very heterogeneous by all methods used thus confirming the view of Leptomonas as an artificial group; 3. The number of current genera of trypanosomatids is not enough to demonstrate the real diversity of these parasites and new genera have to be established.
Mots-clés Agrovoc : parasite, taxonomie, biologie moléculaire, ultrastructure, polymorphisme
Mots-clés complémentaires : Trypanosomatidae
Classification Agris : H10 - Ravageurs des plantes
F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Podlipaev Sergei A., Russian Academy of Science (RUS)
- Frolov A., Russian Academy of Science (RUS)
- Bulat S., Institute of Nuclear Physics (RUS)
- Banuls A.L., ORSTOM [Institut français de recherche scientifique pour le développement en coopération] (FRA)
- Dollet Michel, CIRAD-AMIS-PROTECTION DES CULTURES (USA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/476651/)
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