Assessment of genetic diversity in three subsets constituted from the ICRISAT sorghum collection using random vs non-random sampling procedures : B. Using molecular markers

Grenier Cécile, Deu Monique, Kresovich Stephen, Bramel Cox Paula Jane, Hamon Perla. 2000. Assessment of genetic diversity in three subsets constituted from the ICRISAT sorghum collection using random vs non-random sampling procedures : B. Using molecular markers. Theoretical and Applied Genetics, 101 (1/2) : 197-202.

https://doi.org/10.1007/s001220051469

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Autre titre : Evaluation de la diversité génétique de tois lots de la collection de sorgho de l'ICRISAT par des méthodes d'échantillonnage aléatoire vs. non aléatoire : B. Utilisation de marqueurs moléculaires

Résumé : The large size of the sorghum [Sorghum bi-color (L.) Moench] landrace collection maintained by ICRISAT lead to the establishment of a core collection. Thus, three subsets of around 200 accessions were established from: (1) a random sampling after stratification of the entire landrace collection (L), (2) a selective sampling based on quantitative characters (PCS), and (3) a selection based on the geographical origin of landraces and the traits under farmers' selection (T). An assessment was done of the genetic diversity retained by each sampling strategy using the poly morphisms at 15 microsatellite loci. The landraces of each subset were genotyped with three multiplex polymerase chain reactions (PCRs) of five fluorescent primer-pairs each with semi-automated allele sizing. The average allelic richness for each subset was equivalent (16.1, 16.3 and 15.4 alleles per locus for the subsets PCS, L, and T, respectively). The average genetic diversity was also comparable for the three subsets (0.81, 0.77 and 0.80 for the subsets PCS, L, and T, respectively). Allelic frequency distribution for each subset was compared with a chi-square test but few significant differences were observed. A high percentage of rare alleles (71 to 76% of 206 total rare alleles) was maintained in the three subsets. The global molecular diversity retained in each subset was not affected by a sampling procedure based upon phenotypic characters.

Mots-clés Agrovoc : Sorghum, variété, collection de matériel génétique, échantillonnage, marqueur génétique, variation génétique

Mots-clés complémentaires : Diversité génétique

Classification Agris : F30 - Génétique et amélioration des plantes

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