A method to estimate acetylcholinesterase-active sites and turnover in insects

Charpentier Alice, Menozzi Philippe, Marcel Véronique, Villate François, Fournier Didier. 2000. A method to estimate acetylcholinesterase-active sites and turnover in insects. Analytical Biochemistry, 285 (1) : 76-81.

Résumé : Acetylcholinesterase is the primary target of organophosphorous and carbamate insecticides. Quantitative changes in acetylcholinesterase are suspected to confer resistance to these insecticides, but a method to estimate the amount in insect is not available. A method using irreversible inhibitors has been developed. Among the irreversible inhibitors tested, 7-(methylethoxyphosphinyloxy) -1-methylquinolinium iodide, chlorpyrifos-ethyl-oxon, and coumaphos-oxon were found to be sufficiently potent and specific.

Mots-clés Agrovoc : insecticide, acétylcholinestérase, propriété des pesticides, résistance aux pesticides, lutte anti-insecte, lutte chimique, Drosophila melanogaster, activité enzymatique, inhibition

Classification Agris : H10 - Ravageurs des plantes

Auteurs et affiliations

• Charpentier Alice, Université Toulouse III Paul Sabatier (FRA)
• Menozzi Philippe, CIRAD-CA-COTON (FRA)
• Marcel Véronique, Université Toulouse III Paul Sabatier (FRA)
• Villate François, Université Toulouse III Paul Sabatier (FRA)
• Fournier Didier, Université Toulouse III Paul Sabatier (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/476765/)

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