Montoro Pascal, Teinseree Nongluk, Kongsawadworakul Panida, Michaux-Ferrière Nicole. 2000. Effect of exogenous calcium on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis (rubber tree) friable calli. Plant Cell Reports, 9 (9) : 851-855.
Version publiée
- Anglais
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Autre titre : Effet du calcium exogène sur le transfert de gène par Agrobacterium tumefaciens dans les cals friables d'Hevea brasiliensis
Résumé : The influence of CaCl2 was investigated on Agrobacterium tumefaciens-mediated gene transfer in Hevea brasiliensis friable calli which are usually proliferated on maintenance medium (MM) containing 9 mM CaCl2. Five A. tumefaciens strains (C58pMP90, C58pGV2260, AGL1, LBA4404 and EHA 105) and two binary vectors (pGIN and pCAMBIA2301) were tested and the strain EHA105pC2301 was selected to conduct further experiments. The calli were precultured on MM containing a range of CaCl2 concentrations, then inoculated with Agrobacterium suspension. Transfer of friable calli from MM containing 9 mM CaCl2 to calcium-free medium significantly enhanced the transient bêta-glucuronidase activity. Interestingly, the use of calcium-free Agrobacterium resuspension medium to inoculate friable calli again dramatically increased the transformation efficiency. Induction of Agrobacterium's virulence with acetosyringone remained an important factor to stimulate transformation.
Mots-clés Agrovoc : Hevea brasiliensis, cal, transfert de gène, Agrobacterium tumefaciens, facteur du milieu, calcium, vecteur génétique, milieu de culture, activité enzymatique, callogénèse
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Montoro Pascal, CIRAD-CP-HEVEA (THA)
- Teinseree Nongluk, Department of Agriculture (THA)
- Kongsawadworakul Panida, Mahidol University (THA)
- Michaux-Ferrière Nicole, CIRAD-AMIS-BIOTROP (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/476793/)
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