Efficient protoplast isolation towards an adapted culture protocol for cotton (Gossypium sp.)

Bojinov B., Chupeau M.C., Pannetier Catherine, Chupeau Y.. 2000. Efficient protoplast isolation towards an adapted culture protocol for cotton (Gossypium sp.). In : New frontiers in cotton research : Proceedings of the world cotton research conference - 2, Athens, Greece, September 6-12, 1998. Gillham Fred M. (ed.). Washington : ICAC, 330-334. World Cotton Research Conference. 2, Athènes, Grèce, 6 Septembre 1998/12 Septembre 1998.

Résumé : The media composition for successful cotton (Gossypium sp.) protoplast isolation and cultivation was determined through systematic investigation of all the components of each culture medium. A set of orthogonal design experiments was used to verify the effect of each compound of the initial medium alone and in conibination with other compounds. This allowed defining the optimum compositiono of the media needed to successfully cultivate protoplasts through différent culture stages.

Classification Agris : F60 - Physiologie et biochimie végétale

Auteurs et affiliations

• Bojinov B., Agricultural University of Plovdiv (BGR)
• Chupeau M.C., INRA (FRA)
• Pannetier Catherine, CIRAD-CA-COTON (FRA)
• Chupeau Y., INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/477452/)

Métriques

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