Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso

Solano Philippe, Michel Jean-François, De La Rocque Stéphane, Sidibé Issa, Cuny Gérard, Duvallet Gérard, Cuisance Dominique. 1999. Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso. In : 25th meeting of the international scientific council for trypanosomiasis research and control, Mombasa (Kenya), 27 september-01 october 1999. CSIRTC. s.l. : s.n., Résumé, 1 p. Réunion du Conseil Scientifique International pour la Recherche et la Lutte contre les Trypanosomiases (CSIRLT). 25, Mombasa, Kenya, 27 Septembre 1999/1 Octobre 1999.

Résumé : During a trypanosomosis survey in the agro-pastoral zone of Sideradougou, Burkina Faso, 1036 heads of cattle were examined for trypanosomes using microscopy. The PCR was applied on a subset of 260 buffycoat samples using primers specific for Trypanosoma congolense savannah and riverine-forest groupe, T. vivax, and T. brucei. Parasitological examination and the moloculer technique were compared, showing a better efficiency of the latter. In the near future, the PCR is likely to become an efficient tool to estimate the prevalence of African trypanosomoses in affected areas.

Classification Agris : L72 - Organismes nuisibles des animaux

Auteurs et affiliations

• Solano Philippe, CIRDES (BFA)
• Michel Jean-François, CIRDES (BFA)
• De La Rocque Stéphane, CIRAD-EMVT-ECONAP (BFA)
• Sidibé Issa, CIRDES (BFA)
• Cuny Gérard, IRD (FRA)
• Duvallet Gérard, CIRAD-EMVT (FRA)
• Cuisance Dominique, CIRAD-EMVT-ECONAP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/477717/)

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