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Comparison between c-ELISA and CFT in detecting antibodies to Mycoplasma mycoides mycoides biotype SC in cattle affected by CBPP in Botswana

Amanfu William, Sediadie S., Masupu K.V., Raborokgwe M.V., Benkirane A., Geiger R., Thiaucourt François. 2000. Comparison between c-ELISA and CFT in detecting antibodies to Mycoplasma mycoides mycoides biotype SC in cattle affected by CBPP in Botswana. Annals of the New York Academy of Sciences, 916 : 364-377. Biennial Conference of the Society for Tropical Veterinary Medicine. 5, Key West, États-Unis, 12 Juin 1999/16 Juin 1999.

Article de revue ; Article de revue à facteur d'impact
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Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Histoire et philosophie des sciences; Histoire, histoire de l'art, archéologie; Psychologie-éthologie-ergonomie

Résumé : Contagious bovine pleuropneumonia (CBPP), caused by Mycoplasma mycoides mycoides biotype small colony (SC) (MmmSC) appears to be making a serious comeback in Africa after successful control programs in many parts of the continent during the 1960s and 1970s. Botswana, a country that has been free from the disease for more than 50 years, was affected in 1995. An eradication policy was adopted by the Government of Botswana in which 320,000 cattle in the affected district of Ngamiland, Northwestern Botswana were slaughtered. This was followed by a restocking exercise in which 70,000 cattle were sent to the outbreak areas as replacement stock. It became necessary to carry out serosurveillance in order to ensure that the disease did not reenter Botswana and to ensure that the replacement stock remained free from the disease. The specificity and sensitivity of the complement fixation test (CFT) in Botswana was assessed in 82 cattle affected by the disease and held in a double fenced quarantine camp. The newly developed competitive ELISA was made available to the National Veterinary Laboratory (NVL) through the FAO/IAEA Joint Division in Vienna, Austria. Using postmortem lesions as the gold standard and a 2 x 2 contingency table, the two tests were compared in terms of their sensitivity and specificity in detecting antibodies to MmmSC. The CFT was found to be slightly more sensitive than the c-ELISA, and this could be related to the stage of the disease. A long-term study comparing the progression of the disease with the two tests is, therefore, essential.

Mots-clés Agrovoc : péripneumonie contagieuse bovine, Mycoplasma mycoides, contrôle de maladies, surveillance épidémiologique, anticorps, Test ELISA

Mots-clés géographiques Agrovoc : Afrique au sud du Sahara, Botswana

Mots-clés complémentaires : Mycoplasma mycoides mycoides

Classification Agris : L73 - Maladies des animaux

Auteurs et affiliations

  • Amanfu William, National Veterinary Laboratory (BWA)
  • Sediadie S., National Veterinary Laboratory (BWA)
  • Masupu K.V., Animal Health (BWA)
  • Raborokgwe M.V., Animal Health (BWA)
  • Benkirane A., FAO (ITA)
  • Geiger R., FAO (AUT)
  • Thiaucourt François, CIRAD-EMVT-SANTE ANIMALE (FRA)

Autres liens de la publication

  • Localisation du document : BA_L70 HOU 2834 [(Bibliothèque de Baillarguet)]

Source : Cirad - Agritrop (https://agritrop.cirad.fr/477739/)

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