A highly sensitive, non radioactive assay for T cell activation in cattle : applications in scereening for antigens recognised by CD4+ and CD8+ T cells

Ballingall Keith T., Mwangi Duncan M., MacHugh Niall D., Taracha Evans L.N., Totté Philippe, McKeever Declan J.. 2000. A highly sensitive, non radioactive assay for T cell activation in cattle : applications in scereening for antigens recognised by CD4+ and CD8+ T cells. Journal of Immunological Methods, 239 : 85-93.

https://doi.org/10.1016/S0022-1759(00)00187-3

Article de revue ; Article de revue à facteur d'impact

Version publiée - Anglais
Accès réservé aux personnels Cirad
Utilisation soumise à autorisation de l'auteur ou du Cirad.
478228.pdf
Télécharger (269kB) | Demander une copie

Résumé : We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNy and activation of immune CD4 + and CD8 + T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4 + T cell clones.

Mots-clés Agrovoc : cellule, immunité cellulaire, bovin, antigène, immunologie

Mots-clés complémentaires : Cellule endothéliale bovine

Classification Agris : L50 - Physiologie et biochimie animales

Auteurs et affiliations