Ballingall Keith T., Mwangi Duncan M., MacHugh Niall D., Taracha Evans L.N., Totté Philippe, McKeever Declan J.. 2000. A highly sensitive, non radioactive assay for T cell activation in cattle : applications in scereening for antigens recognised by CD4+ and CD8+ T cells. Journal of Immunological Methods, 239 : 85-93.
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Résumé : We describe a highly sensitive, non-radioactive assay for T cell activation, based on the rapid induction of class II MHC expression by constitutively negative bovine endothelial cells, when cultured in the presence of supernatants derived from activated bovine T cells. We demonstrate the effectiveness of this assay in detecting rBoIFNy and activation of immune CD4 + and CD8 + T cell lines and clones in response to specific antigen and transfected COS-7 cells, respectively. We also demonstrate its utility in identifying purified pathogen fractions that activate immune CD4 + T cell clones.
Mots-clés Agrovoc : cellule, immunité cellulaire, bovin, antigène, immunologie
Mots-clés complémentaires : Cellule endothéliale bovine
Classification Agris : L50 - Physiologie et biochimie animales
Auteurs et affiliations
- Ballingall Keith T., ILRI (KEN)
- Mwangi Duncan M., ILRI (KEN)
- MacHugh Niall D., ILRI (KEN)
- Taracha Evans L.N., ILRI (KEN)
- Totté Philippe, CIRAD-EMVT-SANTE ANIMALE (GLP)
- McKeever Declan J., ILRI (KEN)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/478228/)
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