Results on the characterization and variability of plant trypanosomatids ; some lessons for characterization of mollicutes diagnosed in coconut palms?

Dollet Michel. 2002. Results on the characterization and variability of plant trypanosomatids ; some lessons for characterization of mollicutes diagnosed in coconut palms?. Phytopathology, 92 (6), suppl., Résumé, S119 Annual Meeting of American Phytopathological Society - Caribbean Division. 41, La Havane, Cuba, 11 Juin 2001/15 Juin 2001.

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : In Latin America, besides lethal yellowing, coconuts are affected by other intraphloemic parasites : Phytomonas spp. (Trypanosomatidae). We have performed a characterization of these microorganisms, which involved different steps from serological studies to the amplification and sequencing of particular genes, and progressing from only a few to several isolates. After completion of isoenzymes studies it appeared coconut trypanosomatids (Hartrot) were different from those of oil palm (Marchitez sorpresiva). However recent studies made on the sequences of the SL RNA gene, and 5S rRNA gene showed there were two different groups of trypanosomatids, the first one containing only coconut isolates and the second containing coconut, oil palm, and Alpinia purpurata isolates. But according to a selective sampling- due to various circumstances- we could envisage that group I contained some oil palm isolates if there was the opportunity to succeed in in vitro culturing more oil palm isolates. Using 5S rRNA gene sequences we could conclude that some trypanosomatids found in some European plants were probably introduced from latin America through plant material exchanges. Further characterization, carried out by the seqencing of SL RNA genes, demonstrated that several loci could be amplified with the same primers while it was expected only one sequence in one given trypanosomatid. Studies using the SSU rRNA gene were not found to be more informative. These results brought us some considerations about the investigations carried out on coconut Lethal Yellowing, regarding the world of mollicutes existing in or on plants. (Texte intégral)

Classification Agris : H10 - Ravageurs des plantes

Auteurs et affiliations

• Dollet Michel, CIRAD-EMVT-SANTE ANIMALE (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/480548/)

Métriques

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