Kinetoplast DNA minicircles of phloem-restricted Phytomonas associated with diseases of coconut and oil palms have a two-domain structure

Dollet Michel, Sturm Nancy R., Ahomadegbe J.C., Campbell David A.. 2001. Kinetoplast DNA minicircles of phloem-restricted Phytomonas associated with diseases of coconut and oil palms have a two-domain structure. FEMS Microbiology Letters, 205 : 65-69.

Résumé : We report the cloning and sequencing of the first minicircle from a phloem-restricted, pathogenic Phytomonas sp. (Hart 1) isolated from a coconut palm with hartrot disease. The minicircle possessed a two-domain structure of two conserved regions, each containing three conserved sequence blocks (CSB). Based on the sequence around CSB 3 from Hart 1, PCR primers were designed to allow specific amplification of Phytomonas minicircles. This primer pair demonstrated specificity for at least six groups of plant trypanosomatids and did not amplify from insect trypanosomatids. The PCR results were consistent with a two-domain structure for other plant trypanosomatids.

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Dollet Michel, CIRAD-EMVT-SANTE ANIMALE (FRA)
• Sturm Nancy R., UC (USA)
• Ahomadegbe J.C., Institut Gustave Roussy (FRA)
• Campbell David A., UC (USA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/484847/)

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