Barré Philippe, Layssac Marion, D'Hont Angélique, Louarn Jacques, Charrier André, Hamon Serge, Noirot Michel.
2001. Relationship between parental chromosomic contribution and nuclear DNA content in the coffee interspecific hybrid : Coffea pseudozanguebariae x Coffea liberica var. dewevrei.
In : Dix-neuvième colloque scientifique international sur le café. Actes = 19th International scientific colloquium on coffee. Proceedings ; 19. Internationales wissenschaftliches Kolloquium über Kaffee ; 19° Coloquio cientifico internacional sobre el cafe. ASIC
Résumé : F1 and backcross hybrids were obtained between two diploid coffee species (2n = 22) differing by their nuclear DNA contents (C. pseudozanguebariae (PSE) 2C = 1.13 pg and C. liberica var. dewevrei (DEW) 2C = 1.42 pg). Genomic in situ hybridisation (GISH) and flow cytometry were used on 6 F1 hybrids and 7 backcross hybrids in order to determine their parental chromosomic contribution and their nuclear DNA content (qDNA), respectively. GISH and flow cytometry results in the two species and their interspecific hybrids: 1. GISH efficiently identified chromosomes from both species. 2. In PSE and DEW, two 18S-5.8S-25S rDNA sites were localised on terminal segments of two chromosomes. 3. F1 hybrids had DNA content intermediate between the parental species and contained 11 chromosomes from each species as expected. 4. There was a linear relationship between the number of PSE chromosomes and the nuclear DNA content. Conclusiosn: Flow cytometry allows to sort hybrids with nuclear DNA content and DEW chromosome number close to the cultivated species. In introgression programmes, the aim is to produce plants with the interesting wild trait, but conserving the back-ground of the cultivated species. This is usually done by backcrossing the hybrids having the favourable trait on the cultivated species and this process have to be carried out on about ten generations. This is time-consuming, especially for coffee trees which yield after four years. The use of molecular markers and flow cytometry for early selection could accelerate this pro-cess.
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Barré Philippe, IRD (FRA)
- Layssac Marion, IRD (FRA)
- D'Hont Angélique, CIRAD-CA-Canne à sucre (FRA)
- Louarn Jacques, IRD (CIV)
- Charrier André, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)
- Hamon Serge, IRD (FRA)
- Noirot Michel, IRD (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/485923/)
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