Is acetyl/butyrylcholine specificity a marker for insecticide-resistance mutations in insect acetylcholinesterase ?

Villatte F., Ziliani Philippe, Estrada-Mondaca Sandino, Menozzi Philippe, Fournier Didier. 2000. Is acetyl/butyrylcholine specificity a marker for insecticide-resistance mutations in insect acetylcholinesterase ?. Pest Management Science, 56 (12) : 1023-1028.

Résumé : Substrate specificity has been widely studied in vertebrate cholinesterases and it has been shown that two phenylalanines in the acyl pocket of acetylcholinesterase govern the acceptance of the acetyl/butyryl moiety of the choline esters. As an insecticide-resistance mutation has been evidenced in the acyl pocket of Drosophila melanogaster and Musca domestica acetylcholinesterase we investigated the possibility of linking changes in acetyl/butyrylthiocholine specificity with mutations in insect acetylcholinesterase. We thus analyzed the effect of 28 mutations in Drosophila enzyme on acetyl/butyrylthiocholine, N-methyl/N-propyl-carbamates and ethyl/methyl-paraoxon preference. It appeared that the highest changes on acetyl/butyrylthiocholine and N-propyl/N-methyl-carbamates preference were due to mutations in the acyl pocket. Nevertheless, other insecticide-resistance mutations, not located in the acyl pocket, also modified these substrate preferences. Moreover, the effect of mutations in the acyl pocket was hidden when some other insecticide-resistance mutations were combined in the enzyme. Consequently, acetyl/butyrylthiocholine preference alteration cannot be used as a marker to localize a mutation in the insect AChE.

Mots-clés Agrovoc : résistance aux produits chimiques, insecticide, mutation, choline, Drosophila melanogaster, Musca domestica, acétylcholinestérase

Classification Agris : H10 - Ravageurs des plantes

Auteurs et affiliations

• Villatte F., Institute for Technical Biochemistry (DEU)
• Ziliani Philippe, Université Toulouse III Paul Sabatier (FRA)
• Estrada-Mondaca Sandino, Université Toulouse III Paul Sabatier (FRA)
• Menozzi Philippe, CIRAD-CA-COTON (FRA)
• Fournier Didier, Université Toulouse III Paul Sabatier (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/486151/)

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