Spatial and temporal distribution of geminiviruses in leafhoppers of the genus Cicadulina monitored by conventional and quantitative polymerase chain reaction

Lett Jean-Michel, Granier Martine, Hippolyte Isabelle, Grondin Martial, Royer Monique, Blanc Stéphane, Reynaud Bernard, Peterschmitt Michel. 2002. Spatial and temporal distribution of geminiviruses in leafhoppers of the genus Cicadulina monitored by conventional and quantitative polymerase chain reaction. Phytopathology, 92 (1) : 65-74.

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Spatial and temporal distribution of Maize streak virus (MSV, family Geminiviridae, genus Mastrevirus) was monitored in the vector species Cicadulina mbila and the nonvector species C. chinaï using conventional and real-time quantitative polymerase chain reaction. Sustained feeding on MSV-infected plants showed that virus accumulation reaches a maximum in C. chinaï, but not in C. mbila. After a 3-day acquisition access feeding period (AAP), MSV was detected in the gut, the hernolymph, and the head of C. mbila, but only in the gut of C. chinaï. Similarly, Digitaria streak virus (genus Mastrevirus), which is not transmitted by either of the two species, was only detected in the gut. MSV was detected in the hemolymph of C mbila 3 h after the beginning of the AAP. Although viral DNA progressively decreases in the vector and non vector species after a 3-day AAP, MSV DNA remained stable in the salivary glands of C. nibila.

Mots-clés Agrovoc : virus striure du maïs, virus des végétaux, Cicadulina, distribution tissulaire

Mots-clés complémentaires : Cicadulina mbila, Réaction de polymérisation en chaîne, Cicadulina chinai

Classification Agris : H10 - Ravageurs des plantes
H20 - Maladies des plantes

Auteurs et affiliations

• Lett Jean-Michel, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Granier Martine, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Hippolyte Isabelle, CIRAD-DG-DS (FRA)
• Grondin Martial, CIRAD-AMIS-PROTECTION DES CULTURES (REU)
• Royer Monique, CIRAD-AMIS-PROTECTION DES CULTURES (FRA)
• Blanc Stéphane, INRA (FRA)
• Reynaud Bernard, CIRAD-AMIS-PROTECTION DES CULTURES (REU) ORCID: 0009-0003-6403-3944
• Peterschmitt Michel, CIRAD-AMIS-PROTECTION DES CULTURES (FRA) ORCID: 0000-0001-8709-9418

Source : Cirad - Agritrop (https://agritrop.cirad.fr/486401/)

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