The morphological variability within a population of coffee somatic embryos produced in a bioreactor affects the regeneration and the development of plants in the nursery

Barry-Etienne Dominique, Bertrand Benoît, Schlönvoigt A., Etienne Hervé. 2002. The morphological variability within a population of coffee somatic embryos produced in a bioreactor affects the regeneration and the development of plants in the nursery. Plant Cell, Tissue and Organ Culture, 68 : 153-162.

Résumé : A 1-liter bioreactor was used to obtain approximatively 800 Coffea arabica somatic embryos, 86% of which reached the 'germinated' stage but with morphological heterogeneity. The population was sub-divided into three categories according to cotyledon area: 'small', 'medium' and 'large', that amounted to 32%, 36% and 4.5%, respectively. The effect of embryo morphology on plantlet conversion after direct sowing in soil and on plant development in the nursery was investigated. Somatic embryos with large cotyledons had only a 25% plantlet conversion rate, whereas somatic embryos with small to medium-sized cotyledons had conversion rates of 47% and 63%, respectively. The vigour of the aerial and root systems of regenerated plantlets at the end of the plant conversion stage was also affected as the embryos with small, medium and large cotyledon mostly regenerated small plantlets (0.5-1.5 cm), medium plantlets (1.5-2.5 cm) and large plantlets (2.5-5 cm), respectively. When transplanted in plastic bags, these 3 populations of plantlets exhibited distinct development rates. They had an initial slow growth phase, which was much longer for the small plantlets, followed by a rapid growth phase. After 40 weeks in the nursery, an analysis of the growth parameters of aerial and radical systems showed that the vigour of the plants was strongly related to the vigour of the plantlets transplanted. The heterogeneity of somatic embryos in the bioreactor affected both the plant conversion efficiency in soil and the plant growth in nursery, where it mainly resulted in retarded growth, primarily in plantlets derived from the somatic embryos with small cotyledons.

Mots-clés Agrovoc : Coffea arabica, Embryogénèse somatique, adaptation, développement embryonnaire, embryon somatique, bioréacteur, anatomie végétale, plantule, stade de développement végétal

Classification Agris : F02 - Multiplication végétative des plantes
F62 - Physiologie végétale - Croissance et développement

Auteurs et affiliations

• Barry-Etienne Dominique, CATIE (CRI)
• Bertrand Benoît, CIRAD-CP-CAFE (CRI) ORCID: 0000-0003-1969-3479
• Schlönvoigt A., CATIE (CRI)
• Etienne Hervé, CIRAD-CP-CAFE (FRA) ORCID: 0000-0002-6208-9982

Source : Cirad - Agritrop (https://agritrop.cirad.fr/487948/)

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