Cluster organization of the genes of Streptomyces pristinaespiralis involved in pristinamycin biosynthesis and resistance elucidated by pulsed-field gel electrophoresis

Bamas-Jacques Nathalie, Lorenzon Sophie, Lacroix P., De Swetschin Carole, Crouzet Joël. 1999. Cluster organization of the genes of Streptomyces pristinaespiralis involved in pristinamycin biosynthesis and resistance elucidated by pulsed-field gel electrophoresis. Journal of Applied Microbiology, 87 : 939-948.

Résumé : Streptomyces pristinaespiralis synthesizes pristinamycin, a member of the streptogramin antibiotic family which consists of a mixture of two types of chemically unrelated compounds named pristinamycins I and pristinamycins Il. In order to estimate the size of the Strep. pristinaespiralis chromosome and to elucidate the-organization of the pristinamycin biosynthetic and resistance genes already identified, it was decided to use the pulsed-field gel electrophoresis technique. Results indicate that the Strep. pristinaespiralis chromosome is linear and about 7580 kb, as previously shown for several other Streptomyces species. By hybridization, it could be shown that the biosynthetic and resistance genes for pristinamycins I and pristinamycins II, except for the multidrug resistance gene ptr, are interspersed and seem to be organized as a single large cluster, covering less than 200 kb corresponding to 2-6% of the total size of the chromosome. The consequences and significance of such a genetic organization are discussed.

Mots-clés Agrovoc : antibiotique, résistance génétique, biologie moléculaire, microbiologie

Classification Agris : L50 - Physiologie et biochimie animales

Auteurs et affiliations

• Bamas-Jacques Nathalie, Rhône Poulenc (FRA)
• Lorenzon Sophie, CIRAD-EMVT-SANTE ANIMALE (FRA)
• Lacroix P., Rhône Poulenc (FRA)
• De Swetschin Carole, Rhône Poulenc (FRA)
• Crouzet Joël, Rhône Poulenc (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/508213/)

Métriques

[ Page générée et mise en cache le 2024-11-18 ]