An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia

Segura Sergio D., Coppens D'Eeckenbrugge Géo, Bohorquez Adriana, Ollitrault Patrick, Tohmé Joe. 2002. An AFLP diversity study of the genus Passiflora focusing on subgenus Tacsonia. Genetic Resources and Crop Evolution, 49 (2) : 111-123.

https://doi.org/10.1023/A:1014731922490

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Résumé : DNA variation was studied at the intra- and interspecific levels within the genus #Passiflora#, with particular emphasis on the subgenus #Tacsonia# and its relationship with the subgenus #Passiflora#. AFLP fingerprints were generated for 35 accessions from 21 species, 14 of which belong to subgenus #Tacsonia#, and four to subgenus #Passiflora#. The morphologically intermediate #P. manicata# (subgenus #Manicata#) was also included, as were #P. trinervia# (subgenus #Psylanthus#) and #P. alnifolia# (subgenus #Decaloba#). The two latter were used as outgroup species. Two primer combinations generated 34-80 scorable fragments per genotype for a total of 260 fragments. Polymorphism analyses spanned the levels from individual genotype to section. Subgenus #Tacsonia# presented wide genetic variability at both intra- and interspecific levels, particularly within wild species. The classification based on these markers generally agrees well with current taxonomic definitions of common species, but less with definitions of sections. A geographical component was evident in the intraspecific variation of #P. mixta#, #P. tripartita# var. #mollissima# and #P. tarminiana#, with wider variation in Ecuador and Peru than in Colombia and Venezuela. These same three species. plus #P. cumbalensis#, formed adjacent clusters, showing closer affinity with each other than with the other species of subgenus #Tacsonia#. As expected, #P. manicata# clustered at an intermediate position between subgenera #Tacsonia# and #Passiflora#.

Mots-clés Agrovoc : Passiflora, ressource génétique, marqueur génétique, variation génétique, polymorphisme génétique

Mots-clés géographiques Agrovoc : région andine, Équateur, Pérou, Colombie, Venezuela (République bolivarienne du)

Mots-clés complémentaires : Diversité génétique, Tacsonia

Classification Agris : F30 - Génétique et amélioration des plantes

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