Phage displayed peptides and anti-idiotype antibodies recognised by a monoclonal antibody directed against a diagnostic antigen of Mycoplasma capricolum subsp. capripneumoniae

Benguric Dubravka R., Dungu Baptiste, Thiaucourt François, Du Plessis Dion H.. 2001. Phage displayed peptides and anti-idiotype antibodies recognised by a monoclonal antibody directed against a diagnostic antigen of Mycoplasma capricolum subsp. capripneumoniae. Veterinary Microbiology, 81 (2) : 165-179.

Résumé : A monoclonal antibody (Mab 4.52) raised against Mycoplasma capricolum subsp. capripneumoniae (Mccp) cell lysate was used as a template to obtain substitute antigens recognised by its paratope. Two approaches were investigated: a 17-mer random peptide library displayed on the surface of a filamentous phage was screened by panning on the immobilised Mab 4.52 and anti-idiotype antibodies were generated by immunising a chicken with the F(ab')2 fragments of the antibody. Analysis of the peptide sequences displayed by the isolated phages identified two peptides. Both contained two cysteine residues and had identical or similar amino acids in positions 5 (P), 8 (I/L) and 13 (L). The fusion phages were also recognised by Mab 4.52 in enzyme-linked immunosorbent assay (ELISA) and binding was shown by surface plasmon resonance. One of the peptides was a markedly better inhibitor (67%) of the binding of Mab 4.52 to its original antigen than the other (20%) at 1 mg/ml. After absorption, to remove isotypic and allotypic reactivities, the anti-idiotype lgY was specifically recognised by Mab 4.52 in ELISA and was able to inhibit its binding to the original antigen, whereas anti-idiotype antibodies raised against a bluetongue virus-specific antibody had no effect. In spite of unequivocal binding of the anti-idiotype antibodies and the fusion phages to the paratope of Mab 4.52, goat antisera appeared not to react with either of the surrogate antigens. In contrast, the test sera bound to the original antigen suggesting that Mab 4.52 does not recognise exactly the same antigenic site as antibodies in the goat anti sera.

Classification Agris : L50 - Physiologie et biochimie animales

Auteurs et affiliations

• Benguric Dubravka R., University of Pretoria (ZAF)
• Dungu Baptiste, ARC (ZAF)
• Thiaucourt François, CIRAD-EMVT-SANTE ANIMALE (FRA)
• Du Plessis Dion H., ARC (ZAF)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/510586/)

Métriques

[ Page générée et mise en cache le 2024-12-10 ]