The protein kinases AtMAP3K[epsilon]1 and BnMAP3K[epsilon]1 are functional homologues of Schizosaccharomyces pombe cdc7p and may be involved in cell division

Jouannic Stefan, Champion Anthony, Segui-Simarro José-Maria, Salimova Ekaterina, Picaud Alain, Tregear James, Testillano Pilar, Risueño Maria-Carmen, Simanis Viesturs, Kreis Martin, Henry Yves. 2001. The protein kinases AtMAP3K[epsilon]1 and BnMAP3K[epsilon]1 are functional homologues of Schizosaccharomyces pombe cdc7p and may be involved in cell division. Plant Journal, 26 (6) : 637-649.

Résumé : We identified an Arabidopsis thaliana gene, AtMAP3K(epsilon)1, and a Brassica napus cDNA, BnMAP3K(epsilon)1, encoding functional protein serine/threonine kinases closely related to cdc7p and cdc15p from Schizosaccharomyces pombe and Saccharomyces cerevisiae, respectively. This is the first report of cdc7-related genes in non-fungal eukaryotes; no such genes have as yet been identified in Metazoans. The B. napus protein is able to partially complement a cdc7 loss of function mutation in S. pombe. RT-PCR and in situ hybridisation revealed that the A. thaliana and B. napus genes are expressed in both the sporophytic and the gametophytic tissues of the respective plant species and revealed further that expression is highest in dividing cells. Moreover, AtMAP3K(epsilon)1 gene expression is cell cycle-regulated, with higher expression in G2-M phases. Our results strongly suggest that the plant cdc7p-related protein kinases are involved in a signal transduction pathway similar to the SIN pathway, which positively regulates cytokinesis in S. pombe.

Mots-clés Agrovoc : Arabidopsis thaliana, Brassica napus, localisation de gène, Schizosaccharomyces pombe, Saccharomyces cerevisiae, protéine kinase, cycle cellulaire, mitose, expression des gènes

Classification Agris : F30 - Génétique et amélioration des plantes
F60 - Physiologie et biochimie végétale

Auteurs et affiliations

• Jouannic Stefan, IBP (FRA)
• Champion Anthony, IBP (FRA)
• Segui-Simarro José-Maria, CSIC (ESP)
• Salimova Ekaterina, ISREC (CHE)
• Picaud Alain, IBP (FRA)
• Tregear James, CIRAD-CP-PALMIER (FRA)
• Testillano Pilar, CSIC (ESP)
• Risueño Maria-Carmen, CSIC (ESP)
• Simanis Viesturs, ISREC (CHE)
• Kreis Martin, IBP (FRA)
• Henry Yves, IBP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/511115/)

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