Pareek C.S., Prusinowska I., Walawski K..
2002. Relationship between immuno-relevant lyspzyme gene polymorphism and differentiation of serum lysozyme activity in cattle.
In : Second international symposium on Candidate Genes for Animal Health (C.G.A.H), Montpellier, France, August 16-18th 2002 : abstracts. CIRAD, INRA
Note générale : Session 2 : Mechanisms of defence against infections
Résumé : Properties of serum lysozyme (EC 3.2.1.17) like pH and ionic strength were investigated to identify the relationship between high and low bacteriolytic activities against bacterium Micrococcus lysodeicticus in cattle. The investigation was performed in two previously identified bull's families showing co-segregation of high serum lytic power with mLys-mic-7 marker allele. The results revealed that co-segregating sire's allele mLys-mic-7 show optimum serum lytic activity at pH 6.8 and ionic strength below 0.5, while mLys-mic-3 show the highest serum lytic activity at pH 5.9 with ionic strength 0.1. Three substrates namely, M. lysodeicficus [ML], M. lysodeicticus labeled with a blue dye Remasol brilliant blue R [blueML] and soluble synthetic [rô]-nitrophenyl-penta-N-acetyl-[bêta]-chitopentaoside [PNP-(G1cNAC)5] were utilized to examine the enzymatic and non-enzymatic function of lysozyme. Calorimetric method with blue ML was utilized to determine the degradation action of serum lysozyme on peptidoglycan cell wall. While, calorimetric assay with PNP-(G1cNAc)5 determines the release of [rô]-nitrophenol by lysozyme which allow us to monitor the accurate catalytic reaction of lysozyme on phenylchitinous substrate. Obtained results from both assays show no differentiation of serum lysozyme activity in the analyzed genotype groups. However, serum lytic activity examined by turbidimetric assay using M. lysodeicticus as a substrate, has shown differentiation with respect to mLys-mic-3 and mLys-mic-7 gene variants. It can be therefore concluded that high and low co-segregating mLys-mic alleles in both sires have not enzymatic influence but rather due to the non-enzymatic function. (Texte intégral)
Mots-clés Agrovoc : expression des gènes, infection, mécanisme de défense
Classification Agris : L10 - Génétique et amélioration des animaux
Auteurs et affiliations
- Pareek C.S., University of Warmia and Mazury (POL)
- Prusinowska I., University of Warmia and Mazury (POL)
- Walawski K., University of Warmia and Mazury (POL)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/512036/)
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