Candidate genes and response to Salmonella enteritidis challenge or vaccination in young chicks

Lamont S.J., Liu W.. 2002. Candidate genes and response to Salmonella enteritidis challenge or vaccination in young chicks. In : Second international symposium on Candidate Genes for Animal Health (C.G.A.H), Montpellier, France, August 16-18th 2002 : abstracts. CIRAD, INRA. Montpellier : CIRAD, Résumé, 1 p. International Symposium on Candidate Genes for Animal Health. 2, Montpellier, France, 16 August 2002/18 August 2002.

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Additional Information : Session 3 : Genetic resistance / susceptibility to infectious diseases (bacteria / virus)

Abstract : Salmonella enteritidus (SE) contamination of poultry products is of global food-safety concern. Several candidate genes were selected to study the genetic control of chicken response to SE, based on the genes' critical roles in host response to intracellular bacteria. Some of these genes - the natural resistance-associated macrophage protein 1 (NRAMP1) and the major histocompatibility complex (MHC) class I and class II - had been previously demonstrated in other populations with different Salmonella-response measures to be associated with Salmonella response. Other genes, including prosaposin, caspase-1, and inhibitor of apoptosis protein-1 (IAP1) genes, had not previously been characterized for activity in SE response. Responses to pathogenic SE colonization or to SE vaccination were evaluated in the Iowa Salmonella Response Resource Population (ISRRP). Outbred broiler sires and three diverse, highly inbred dam lines (two MHC-congenic Leghorn line and one Fayoumi line) produced 508 F1 progeny, which were evaluated as young chicks for either bacterial load isolated from samples taken from the spleen and cecum after pathogenic SE inoculation, or circulating antibody level after SE vaccination. Fragments of each gene were sequenced from the founder lines of the resource population to identify genomic sequence variation. Then, PCR-RFLP, BESS-T Scan, or SSCP techniques were applied to genotype the SNPs in the F1 resource population for each candidate gene, Linear mixed models were used for statistical analyses. Because the inbred dam lines always contributed one copy of the same allele, the heterozygous sire allele effects could be assessed in the F1 generation. Broiler sire alleles of NRAMP1, MHC class I and class II, caspase-1, IAP1, and prosaposin genes were associated with spleen bacterial load. Broiler sire alleles of NRAMP1 and caspase-1 were associated with cecum content bacterial load and antibody response to SE vaccine. The unique population design also allowed detection of gene interactions between sire allele and other genetic factors, such as dam line and sex. These interactions were frequently significant. This is the first reported study on the association between caspase-1, IAP1, and prosaposin gene polymorphisms and chicken response to SE. It also extends the knowledge on associations of the MHC and NRAMP1 gene effects on Salmonella response by evaluating new resource populations and new aspects to response to SE. Identification of candidate genes for improved immune response may be very useful for marker-assisted selection to enhance disease resistance. (Texte intégral)

Mots-clés Agrovoc : Salmonella enteritidis, Vaccination, Poulet

Classification Agris : L10 - Animal genetics and breeding

Auteurs et affiliations

  • Lamont S.J., Iowa State University (USA)
  • Liu W., Iowa State University (USA)

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