Comparative proteome analysis of the porcine RN mutation in PRKG3

Hedegaard J., Horn P., Lametsch R., Roepstorff P., Bendixen Christian, Bendixen E.. 2002. Comparative proteome analysis of the porcine RN mutation in PRKG3. In : Second international symposium on Candidate Genes for Animal Health (C.G.A.H), Montpellier, France, August 16-18th 2002 : abstracts. CIRAD, INRA. Montpellier : CIRAD, Résumé, 1 p. International Symposium on Candidate Genes for Animal Health. 2, Montpellier, France, 16 August 2002/18 August 2002.

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Additional Information : Session 4 : Genetic resistance / susceptibility to non-infectious diseases

Abstract : The PRKAG3 gene encodes a muscle specific isoform of the regulatory [gamma]3 subunit of the AMP-activated kinase (AMPK). AMPK acts as a metabolic sensor, which in its activated form inhibits ATP consuming pathways and stimulates ATP generating pathways. A dominant mutation in the porcine PRKAG3 gene, namely the RNmutation, leads to a ~ 70% increase in the glycogen content of glycolytic muscle cells. This is converted into lactate postmortem resulting in a lowered ultimate pH, severly affecting the meat quality and processing yields. To study how this mutation influences the protein expression of skeletal muscle tissues, two dimensional electrophoresis, digital image analysis and MaIdi-TOF based identification of differentially expressed proteins was performed on samples from 23 animals (11 rn+/rn+ and 12 rn+/ RN-). Several protein spots were found to differ significantly in integrated intensity dependent on genotype and the most notable differences were verified by means of enzymatic assays and Northern blot analysis. The proteins were by Maldi-TOF identified as essential enzymes in the synthesis of glycogen as well as enzymes from the glycolysis and the Krebs cycle. This is in agrement with the finding that the RN-mutation results in an increased level of muscle glycogen and a more oxidative metabolism. The molecular link between the RN-mutation in AMPK and the various observed effects has not yet been described. (Texte intégral)

Mots-clés Agrovoc : Analyse biologique, Porcin, Mutation homéotique, Mutagénèse

Classification Agris : L10 - Animal genetics and breeding

Auteurs et affiliations

  • Hedegaard J., Danish Institute of Agricultural Sciences (DNK)
  • Horn P., Danish Institute of Agricultural Sciences (DNK)
  • Lametsch R., Danish Institute of Agricultural Sciences (DNK)
  • Roepstorff P., University of Southern Denmark (DNK)
  • Bendixen Christian, Danish Institute of Agricultural Sciences (DNK)
  • Bendixen E., Danish Institute of Agricultural Sciences (DNK)

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