Sallaud Christophe.
2000. Organization of t-DNA integration in a large population of transgenic rice.
Résumé : A large number of transgenic rice plants have been generated using Agrobacterium strain EHA 105 harboring a pCAMBIA binary vector having a gus and gfp reporter gene under the control of the CaMV-35S promoter. Histochemical detection of GUS activity and GFP fluorescence have been found in 40 to 80% of the plants depending the organ and/or the development stage of the tested plants. Molecular analysis have been performed on 117 independent transgenic plants to examine (i) the percentage of T-DNA rearrangement (ii) whether a correlation between the structure of T-DNA integration and the expression of the two reporter genes could exist in this population. First results indicate that 33 transformants (29%) contain a single intact T-DNA copy, 26 (22%) transformants a non T-DNA sequence corresponding to the binary vector backbone, 21 (18%) and 17 (14%) transformants a gus or gfp truncated or missing cassette respectively. GUS and GFP assay performed on leaves of T0 and T1 transgenic plants indicate clearly that the percentage of transformants expressing the gus gene is higher when only one copy of the gene is integrated into the genome. (Texte intégral)
Mots-clés Agrovoc : Oryza, plante transgénique, Agrobacterium tumefaciens
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Sallaud Christophe, CIRAD-AMIS-BIOTROP (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/512293/)
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