Legume grains enhance ileal losses of specific endogenous serine-protease proteins in weaned pigs

Salgado Paulo, Montagne Lucie, Freire Joao P.B., Ferreira Ricardo B., Teixeira Artur R., Bento Ofélia, Abreu Manuel C., Toullec René, Lallès Jean-Paul. 2002. Legume grains enhance ileal losses of specific endogenous serine-protease proteins in weaned pigs. Journal of Nutrition, 132 (7) : 1913-1920.

Résumé : Feeding legume grains to pigs usually increases losses of endogenous proteins at the terminal ileum. However, the identity of such proteins is largely unknown. This study was undertaken to determine the ileal flow and identity of soluble proteins present in large concentrations in ileal digesta of young pigs fed soybean meal (SBM), peas (P), faba beans (FB), or blue lupin (L) in expt. 1, and white (WPC) or black (BPC) chickpeas in expt. 2. Protein in the control diet (C) was provided by casein. Ileal digesta proteins were analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis, Coomassie blue staining, densitometry and N-terminal amino acid sequencing. Three protein bands at molecular masses of 25, 27, and 30 kDa had a higher ileal flow (P < 0.05) in the pigs fed the legume-based diets compared to those fed the control diet in expt. 2. This was true for the 25- and 30-kDa proteins (P < 0.05) and the 27-kDa protein (P < 0.10) in pigs fed the legume-containing diets in expt. 1. These proteins shared N-terminal amino acid sequences with enzymes of the serine protease family including pig trypsin (25 kDa) and blood coagulation factor IX or chymotrypsin (27 and 30 kDa).

Classification Agris : L51 - Physiologie animale - Nutrition

Auteurs et affiliations

• Salgado Paulo, INRA (FRA)
• Montagne Lucie
• Freire Joao P.B., Universidade Técnica de Lisboa (PRT)
• Ferreira Ricardo B., Universidade Técnica de Lisboa (PRT)
• Teixeira Artur R., Universidade Técnica de Lisboa (PRT)
• Bento Ofélia, Universidade de Evora (PRT)
• Abreu Manuel C., Universidade de Evora (PRT)
• Toullec René, INRA (FRA)
• Lallès Jean-Paul, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/512517/)

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