Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a srological marker for experimental and natural infection

Truong Catherine, Mahé Dominique, Blanchard Philippe, Le Dimna Mireille, Madec François, Jestin André, Albina Emmanuel. 2001. Identification of an immunorelevant ORF2 epitope from porcine circovirus type 2 as a srological marker for experimental and natural infection. Archives of Virology, 146 (6) : 1197-1211.

Résumé : Post-weaning multisystemic wasting syndrome (PMWS) is a recently identified disease of pigs linked to the emergence of a new porcine circovirus (PCV2). We report here the characterization of immunorelevant linear B-cell epitopes of the Open Reading Frame 2-encoded protein (Orf2) from PCV2 by an enzyme-linked immunosorbent assay (ELISA) using experimental antisera collected from pigs inoculated with a PCV2 isolate. Two epitopes spanning residues 69 to 83 and 117 to 131 were specific to PCV2. Antibodies to the 117 to 131 epitope (B-133) were detected in 22% and 100% of specific pathogen-free (SPF) pig sera 6 and 11 weeks post inoculation, respectively. Cross-sectional studies performed with field sera collected from PMWS-affected herds showed B-133 antibodies in 5% of 8 to 10 week-old pigs, 38% of 13-14 week-old pigs, 62% of 16 to 19 week-old pigs, 56% of 20 to 25 week-old pigs and 45% of 26 to 31 week-old pigs. All these data suggest that epitope B-133 is a serological marker of PCV2 infection that could be used for the detection of PCV2 antibody response.

Classification Agris : L50 - Physiologie et biochimie animales

Auteurs et affiliations

• Truong Catherine, AFSSA (FRA)
• Mahé Dominique, AFSSA (FRA)
• Blanchard Philippe, AFSSA (FRA)
• Le Dimna Mireille, AFSSA (FRA)
• Madec François, AFSSA (FRA)
• Jestin André, AFSSA (FRA)
• Albina Emmanuel, AFSSA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/512833/)

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