Production of haploids from anther culture of banana (Musa balbisiana (BB))

Assani A., Bakry Frédéric, Kerbellec Florence, Haïcour R., Wenzel Gerhard, Foroughi-Wehr Barbel. 2003. Production of haploids from anther culture of banana (Musa balbisiana (BB)). Plant Cell Reports, 21 (6) : 511-516.

https://doi.org/10.1007/s00299-002-0557-6

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Résumé : We report here, for the first time, the production of haploid plants of banana #Musa balbisiana# (BB). Callus was induced from anthers in which the majority of the microspores were at the uninucleate stage. The frequency of callus induction was 77%. Callus proliferation usually preceded embryo formation. About 8% of the anthers developed androgenic embryos. Of the 147 plantlets obtained, 41 were haploids (n=x=11). The frequency of haploid production depended on genotypes used: 18 haploid plants were produced from genotype 'Pisang klutuk', 12 from 'Pisang batu', seven from 'Pisang klutuk wulung' and four from 'Tani'. The frequency of regeneration was 1.1%, which was based on the total number of anthers cultured. Diploid plants (2n=2x=22) were also observed in the regenerated plants. The haploid banana plants that were developed will be important material for the improvement of banana through breeding programmes.

Mots-clés Agrovoc : Musa balbisiana, haploïdie, culture d'anthère, androgénèse, régénération in vitro, nombre chromosomique, amélioration des plantes

Mots-clés complémentaires : Régénération de plante

Classification Agris : F02 - Multiplication végétative des plantes
F30 - Génétique et amélioration des plantes

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