Analysis of latex expressed sequence tags (ESTs) in Hevea brasiliensis

Chow Keng See, Sunderasan E., Tan Siang He, Harikrishna K., Yeang Hoong Yeet. 2001. Analysis of latex expressed sequence tags (ESTs) in Hevea brasiliensis. In : Biotechnology and rubber tree : Proceedings of IRRDB symposium, 25-28 September 2001, Montpellier, France. Sainte-Beuve Jérôme (ed.). IRRDB, CIRAD-CP-HEVEA. Montpellier : CIRAD IRRDB Symposium, Montpellier, France, 25 Septembre 2001/28 Septembre 2001.

Résumé : Natural rubber from Hevea brasiliensis latex consists of a high molecular weight polymer composed of isopentenyl diphosphate (IPP) molecules. Latex is the cytoplasmic fluid contained in the vessel tubes or laticifers which are located in the bark of the Hevea tree. As cytoplasm, latex would be expected to contain general cellular processes carried out by eukaryotic cells and more specifically, rubber biosynthesis. In this project, we are interested in building a profile of genes expressed in latex. These expressed genes or Expressed Sequence Tags (ESTs) are obtained by sequencing random cDNA clones which represent the genes transcribed in latex. Our objective is to generate a pool of Hevea ESTs which would serve as a molecular genetics resource to facilitate various aspects of rubber research. Furthermore, an EST library will also create the way for microarray-based studies of Hevea gene expression. In this study, we report the current status of EST sequencing of clones from a latex cDNA library.

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Chow Keng See, MRB (MYS)
• Sunderasan E., MRB (MYS)
• Tan Siang He, Institute of Bioscience (MYS)
• Harikrishna K., Institute of Bioscience (MYS)
• Yeang Hoong Yeet, MRB (MYS)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/513737/)

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