Effect of cytokinins on in vitro germination of Hevea somatic embryos

Kumari Jayasree P., Sunithra S.S., Thulaseedaran A.. 2001. Effect of cytokinins on in vitro germination of Hevea somatic embryos. In : Biotechnology and rubber tree : Proceedings of IRRDB symposium, 25-28 September 2001, Montpellier, France. Sainte-Beuve Jérôme (ed.). IRRDB, CIRAD-CP-HEVEA. Montpellier : CIRAD IRRDB Symposium, Montpellier, France, 25 Septembre 2001/28 Septembre 2001.

Résumé : In Hevea brasiliensis somatic embryogenesis may offer new possibilities for crop improvement programs via. genetic transformation when the other in vitro techniques remain difficult. In the recent past, although substantial progress has been made in Hevea somatic embryogenesis, the embryo germination and subsequent development into viable plantlets still remain an enigma for most of the researchers worldwide. A detailed investigation on the response of various cytokinins on germination was carried out using benzyl adenine (BA), zeatin (ZEA), kinetin (KIN) and thidiazuron (TDZ). Irrespective of the type and concentration of cytokinins used, germination started within 7-10 days of transfer to plant regeneration medium. TDZ proved to be superior to BA and ZEA while KIN showed the least response. 70% germination and 83% plant development occurred with BA while ZEA resulted in 67% germination and 80% plant recovery. Maximum germination and plant development (80% and 82% respectively) was obtained when the medium was supplemented with TDZ. A low germination rate of 53% was obtained with KIN. The initial growth of plantlets in terms of height, shoot length and number of leaves was higher for plantlets produced with TDZ when compared to BA and ZEA derived plants. In the present paper an efficient method for achieving a high frequency germination and plant recovery from somatic embryos have been described for the clone RRII 105 using immature anthers as explants.

Classification Agris : F02 - Multiplication végétative des plantes

Auteurs et affiliations

• Kumari Jayasree P., RRII (IND)
• Sunithra S.S., Mahatma Gandhi University (IND)
• Thulaseedaran A., RRII (IND)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/513741/)

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