Laser diffraction : A new tool for identification and studies of the physiological effectors involved in aggregation-coagulation of rubber particles from hevea latex

Chrestin Hervé, Kongsawadworakul Panida, Laurent Jean Yves, Noirot Michel. 2001. Laser diffraction : A new tool for identification and studies of the physiological effectors involved in aggregation-coagulation of rubber particles from hevea latex. In : Biotechnology and rubber tree : Proceedings of IRRDB symposium, 25-28 September 2001, Montpellier, France. Sainte-Beuve Jérôme (ed.). IRRDB, CIRAD-CP-HEVEA. Montpellier : CIRAD IRRDB Symposium, Montpellier, France, 25 Septembre 2001/28 Septembre 2001.

Résumé : Coagulation of latex is the first limiting factor of rubber yield in Hevea brasiliensis. Using laser diffraction, we set up a new method allowing to monitor the kinetics of rubber particles (RP) aggregation. Contrary to the previous methods used (visual observation and more recently spectrophotometry), laser diffraction allows to continuously follow the changes in size of RP and aggregates, then to quantify and characterize the phenomena involved in the aggregation-coagulation processes. Using this technique we confirmed that the major RP aggregating factors are proteins compartmentalized within the latex cells vacuoles. Opposite, the latex cytosol is confirmed to harbour anti-aggregating proteins. Lutoidic serum alone can induce formation of RP loose aggregates which can be large enough to provoke the obstruction of the severed latex vessels extremities, but not irreversible RP coagulation. Using this new method, identification and characterization of the other proteins, as well as their corresponding genes, involved (as +/- effectors) in latex destabilisation/coagulation, hence in rubber yield, are on the process.

Classification Agris : F60 - Physiologie et biochimie végétale
U30 - Méthodes de recherche
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Chrestin Hervé, IRD (FRA)
• Kongsawadworakul Panida, Mahidol University (THA)
• Laurent Jean Yves
• Noirot Michel, IRD (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/513750/)

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