Expression of functional recombinant antibody fragment in the latex of transgenic Hevea brasiliensis

Yeang Hoong Yeet, Arokiaraj P., Jaafar H., Arif S.A.M., Rajamanikam S., Chan J.L., Sharib J., Leelavathy R., Hamzah S.. 2001. Expression of functional recombinant antibody fragment in the latex of transgenic Hevea brasiliensis. In : Biotechnology and rubber tree : Proceedings of IRRDB symposium, 25-28 September 2001, Montpellier, France. Sainte-Beuve Jérôme (ed.). IRRDB, CIRAD-CP-HEVEA. Montpellier : CIRAD IRRDB Symposium, Montpellier, France, 25 Septembre 2001/28 Septembre 2001.

Résumé : Hevea brasiliensis produces voluminous latex which can be extracted non-destructively through the process of tapping. By transforming Hevea with genes that control the production of high-value proteins, transgenic rubber plants could serve as efficient, low cost, low maintenance and environment-friendly production lines for the production of the targeted protein. The transgenic rubber tree then becomes essentially a living ¿factory¿. The system would enable continual harvesting of the protein exuded in the latex that is free of bacteria and animal viruses. As Hevea is amenable to vegetative propagation, large numbers of genetically identical plants can be multiplied from a single selected transformant. Examples of commercially valuable proteins are those employed in pharmacology and personal care products. One of the genes that has been transformed into Hevea encoded an antibody single chain variable fragment (scFv) against the coat protein of the bacterium Streptococcus sanguis. This recombinant antibody fragment has been successfully synthesised in plants, but extraction and recovery of the target protein remained a problem because of the low yield and the fact that destructive harvesting was involved1.

Auteurs et affiliations

• Yeang Hoong Yeet, MRB (MYS)
• Arokiaraj P., MRB (MYS)
• Jaafar H., MRB (MYS)
• Arif S.A.M., MRB (MYS)
• Rajamanikam S., MRB (MYS)
• Chan J.L., MRB (MYS)
• Sharib J., MRB (MYS)
• Leelavathy R., MRB (MYS)
• Hamzah S., MRB (MYS)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/513753/)

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