AFLP and SSR polymorphism in a Coffea interspecific backcross progeny ((Coffea heterocalyx X Coffea Canephora) X C. Canephora) [S10-45]

Coulibaly Issa, Revol Brigitte, Noirot Michel, Poncet Valérie, Lorieux Mathias, Carasco-Lacombe Catherine, Minier Jérôme, Hamon Perla. 2003. AFLP and SSR polymorphism in a Coffea interspecific backcross progeny ((Coffea heterocalyx X Coffea Canephora) X C. Canephora) [S10-45]. In : 7th International Congress of Plant Molecular Biology, ISPMB 2003, Barcelona (Spain), June 23-28. Book of abstracts. ISPMB. Barcelone : ISPMB Office, Résumé, 152. International Congress of Plant Molecular Biology. 7, Barcelone, Espagne, 23 Juin 2003/28 Juin 2003.

Résumé : An interspecific cross (BC 1) involving a species with one of the biggest genomes in Coffea genus (C. heterocalyx (HET), qDNA = 1.74 pg) and a species with a medium genome size (C. canephora (CAN), qDNA = 1.43 pg) was studied using two types of molecular markers, AFLPTM and SSR. One hundred and eighty eight AFLP bands and 34 SSR primer pairs were suitable for the mapping. The total map length was 1360 cM with 190 loci distributed on 15 linkage groups. The results were compared to the results obtained previously on an interspecific BC 1 progeny involving a species with a medium size genome (C. liberica var dewevrei, DEW) and a species with one of the smallest size genome (C. pseudozanguebariae, PSE). They are discussed considering three main points: 1) the interest of the different markers type, 2) - the genomic distribution of AFLPs and SSR markers and 3) - the relation between AFLP polymorphism and the genome size. (Texte intégral)

Mots-clés Agrovoc : Coffea, Coffea canephora, hybridation interspécifique, épreuve sur la descendance, polymorphisme génétique, marqueur génétique, carte génétique

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Coulibaly Issa, CIRAD-CP-UMR DGPC (FRA)
• Revol Brigitte, CIRAD-CP-UMR DGPC (FRA)
• Noirot Michel, IRD (FRA)
• Poncet Valérie, CIRAD-CP-UMR DGPC (FRA)
• Lorieux Mathias, CNRS (COL)
• Carasco-Lacombe Catherine, CIRAD-CP-CAFE (FRA)
• Minier Jérôme, CIRAD-CP-UMR DGPC (FRA) ORCID: 0000-0002-5993-4663
• Hamon Perla, CIRAD-CP-UMR DGPC (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/514051/)

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