Soussi M., Escoute Jacques, Payre H., Drevon Jean Jacques.
2003. Hybridation in situ d'un gène d'aquaporine dans les nodosités de haricot (Phaseolus vulgaris) BAT477.
In : Fixation symbiotique de l'azote et développement durable dans le bassin méditerranéen : Carthage (Tunisie), 13-16 octobre 1998, Montpellier (France), 9-13 juillet 2000. Drevon Jean Jacques (ed.), Sifi B. (ed.). Groupe FABAMED, INRA
Résumé : Aquaporins are water-channel proteins. In plants they are very diversified, and found in plasmalemic and tonoplastic membranes in numerous organs, including the peribacteroid membrane of N2-fixing nodules in legumes. In this work we have examined whether the expression of an aquaporin gene is tissue-specific within Phaseolus vulgaris nodules and how it relates with its N2-fixing activity. Common bean (BAT477) seeds were surface sterilized and inoculated with Rhizobium tropici CIAT899, then grown hydroaeroponically in glasshouse. Fully active nodules of 3 mm diameter were collected at 35 days and immediately cut in half, fixed and subsequently embedded in methacrylate resin for making 5 µm sections. In situ hybridisation was performed with 35 S radio-labeled RNA probe of a Mip-1 aquaporin DNA fragment obtained by RT-PCR from a RNA extract of BAT477 nodule-cortex. There was no signal with the sense probe. With the antisense probe the results show a much higher density of the radioactive signal in the cortex parenchyma than in the infected zone. In the later, the signal was found only in the non-infected cells. Within the cortex, the signal was higher in the 2-4 cell layers between the vascular traces and the infected zone, i.e. the innercortex, than in the next 2-3 surrounding cell layers, i.e. the middle-cortex. In addition, the signal was surprisingly high in the first 2 cell-layers surrounding the later. An increase in the inner-cortex signal was associated with salinity.
Mots-clés Agrovoc : Phaseolus vulgaris, biologie, analyse histocytologique, Rhizobium, symbiose
Mots-clés complémentaires : Rhizobium tropici
Classification Agris : F60 - Physiologie et biochimie végétale
Auteurs et affiliations
- Soussi M., INRA (FRA)
- Escoute Jacques, CIRAD-AMIS-BIOTROP (FRA)
- Payre H., INRA (FRA)
- Drevon Jean Jacques, INRA (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/514526/)
[ Page générée et mise en cache le 2024-03-25 ]
