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Hybridation in situ d'un gène d'aquaporine dans les nodosités de haricot (Phaseolus vulgaris) BAT477

Soussi M., Escoute Jacques, Payre H., Drevon Jean Jacques. 2003. Hybridation in situ d'un gène d'aquaporine dans les nodosités de haricot (Phaseolus vulgaris) BAT477. In : Fixation symbiotique de l'azote et développement durable dans le bassin méditerranéen : Carthage (Tunisie), 13-16 octobre 1998, Montpellier (France), 9-13 juillet 2000. Drevon Jean Jacques (ed.), Sifi B. (ed.). Groupe FABAMED, INRA. Paris : INRA, pp. 401-410. (Les colloques, 100) ISBN 2-7380-1065-2 Colloque sur la fixation symbiotique de l'azote et développement durable dans le Bassin méditerranéen, Carthage, Tunisie, 13 October 1998/16 October 1998.

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Abstract : Aquaporins are water-channel proteins. In plants they are very diversified, and found in plasmalemic and tonoplastic membranes in numerous organs, including the peribacteroid membrane of N2-fixing nodules in legumes. In this work we have examined whether the expression of an aquaporin gene is tissue-specific within Phaseolus vulgaris nodules and how it relates with its N2-fixing activity. Common bean (BAT477) seeds were surface sterilized and inoculated with Rhizobium tropici CIAT899, then grown hydroaeroponically in glasshouse. Fully active nodules of 3 mm diameter were collected at 35 days and immediately cut in half, fixed and subsequently embedded in methacrylate resin for making 5 µm sections. In situ hybridisation was performed with 35 S radio-labeled RNA probe of a Mip-1 aquaporin DNA fragment obtained by RT-PCR from a RNA extract of BAT477 nodule-cortex. There was no signal with the sense probe. With the antisense probe the results show a much higher density of the radioactive signal in the cortex parenchyma than in the infected zone. In the later, the signal was found only in the non-infected cells. Within the cortex, the signal was higher in the 2-4 cell layers between the vascular traces and the infected zone, i.e. the innercortex, than in the next 2-3 surrounding cell layers, i.e. the middle-cortex. In addition, the signal was surprisingly high in the first 2 cell-layers surrounding the later. An increase in the inner-cortex signal was associated with salinity. (Résumé d'auteur)

Mots-clés Agrovoc : Phaseolus vulgaris, Biologie, Analyse histocytologique, Rhizobium, Symbiose

Mots-clés complémentaires : Rhizobium tropici

Classification Agris : F60 - Plant physiology and biochemistry

Auteurs et affiliations

  • Soussi M., INRA (FRA)
  • Escoute Jacques, CIRAD-AMIS-BIOTROP (FRA)
  • Payre H., INRA (FRA)
  • Drevon Jean Jacques, INRA (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/514526/)

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