Studies on the safety and immunogenicity of the South African bluetongue virus serotype 2 monovalent vaccine : specific detection of the vaccine strain genome by RT-PCR

Hammoumi Saliha, Breard Emmanuel, Sailleau Corinne, Russo Pierre, Grillet Colette, Cetre-Sossah Catherine, Albina Emmanuel, Sanchis R., Pépin Michel, Zientara Stéphan. 2003. Studies on the safety and immunogenicity of the South African bluetongue virus serotype 2 monovalent vaccine : specific detection of the vaccine strain genome by RT-PCR. Journal of Veterinary Medicine. Series B, 50 : 316-321.

https://doi.org/10.1046/j.1439-0450.2003.00682.x

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Résumé : In order to study the safety and the immunogenicity of the South African vaccine against the serotype 2 bluetongue virus, two groups of seven sheep were vaccinated with the vaccine used in the French island of Corsica. Vaccinated and nonvaccinated sheep were observed clinically and their rectal temperatures were recorded daily. The serological response in vaccinated animals confirmed the immunogenicity of the vaccine. Post-vaccinal viraemia was investigated and the vaccine genome was detected by reverse transcription polymerase chain reaction (RT-PCR). No viraemia was observed at postvaccination days 4, 7 and 11 but the vaccine strain of virus was detected by RT-PCR throughout the experiment. The thermostability of the vaccine was also evaluated. The vaccine titre strongly decreased at temperatures higher than 35°C.

Mots-clés Agrovoc : fièvre catarrhale ovine, vaccin, sérotype, réponse immunitaire

Mots-clés géographiques Agrovoc : Corse, France

Classification Agris : L73 - Maladies des animaux

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