Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins

Blanchard Philippe, Mahé Dominique, Cariolet Roland, Keranflec'h A., Baudouard M.A., Cordioli P., Albina Emmanuel, Jestin André. 2003. Protection of swine against post-weaning multisystemic wasting syndrome (PMWS) by porcine circovirus type 2 (PCV2) proteins. Vaccine, 21 : 4565-4575.

Résumé : Porcine circovirus type 2 (PCV2) is known to be associated with post-weaning multisystemic wasting syndrome (PMWS), a recently described disease of young pigs. Since no PCV2 vaccine was available so far, we have developed a specific PCV2 vaccine candidate. The Orf1-encoded replication protein and Orf2-encoded capsid protein of PCV2 were expressed and detected in either mammalian or insect expression systems. In a first trial, Orf2 protein was found to be a major immmogen, inducing protection in a prime-boost protocol; the piglets received a first injection with plasmids directing Orf2 protein and granulocyte-macrophage colony-stimulating factor (GM-CSF) expression, followed by a second injection, a fortnight later, associated with baculovirus-expressed Orf2 protein. As evaluated by growth parameters, clinical signs (fever), seroconversion, the pigs were protected against a PCV2 challenge after vaccination. In a second trial, protection induced by a subunit vaccine was even better than the one induced by DNA vaccine, since PCV2 replication was completely inhibited.

Mots-clés Agrovoc : porcin, vaccin, Circoviridae

Classification Agris : L73 - Maladies des animaux

Auteurs et affiliations

• Blanchard Philippe, AFSSA (FRA)
• Mahé Dominique, AFSSA (FRA)
• Cariolet Roland, AFSSA (FRA)
• Keranflec'h A., AFSSA (FRA)
• Baudouard M.A., LDA (FRA)
• Cordioli P., IZSLER (ITA)
• Albina Emmanuel, CIRAD-EMVT-SANTE ANIMALE (FRA)
• Jestin André, AFSSA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/517234/)

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