Dollet Michel, Sturm Nancy R., Campbell David A..
2003. Internal transcribed spacer of ribosomal RNA genes and microsatellites for characterization of phloem restricted Phytomonas associated with coconut and oil palm wilts.
In : Pan American Plant disease Conference : 42nd Annual meeting on the American Phytopapthological Society-Caribbean Division, South Padre Island, Texas, U.S.A, April 6-10, 2003
Résumé : Phloem-restricted trypanosomatids - Phytomonas - are associated with wilts of oil palm, coconut, coffee, red ginger in Latin America and the Caribbean. The coconut wilt -Hartrot- and Lethal Yellowing phytoplasma disease- have the same symptomatology and can be confused. As similar non-pathogenic trypanosomatids can also be found in plants and insects it is important to have specific tools to diagnose the strains associated with wilts. Here we examine the internal transcribed spacer (ITS) region of the rRNA locus. It contains two distinct ITSs separated by the 5.8S rRNA gene. The ITS - 5.8 S region is a robust marker for characterization of the different types of trypanosomatids and distinguishes the phloem-restricted ones. Microsatellite sequences were identified for the first time in Phytomonas in ITS regions. Several classes were seen, with interisolate variation that has potential for future use in population genetics studies. (Texte intégral)
Mots-clés Agrovoc : Trypanosoma, Phytomonas, Cocos nucifera, génétique
Classification Agris : F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Dollet Michel, CIRAD-EMVT-SANTE ANIMALE (FRA)
- Sturm Nancy R., UCLA (USA)
- Campbell David A., UCLA (USA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/517445/)
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