Quantification of mucin in ileal digesta of piglets using a new anzyme-linked immunosorbent assay

Piel Christelle, Montagne Lucile, Salgado Paulo, Lallès Jean-Paul. 2003. Quantification of mucin in ileal digesta of piglets using a new anzyme-linked immunosorbent assay. In : Proceedings of the 9th International symposium on digesctive physiology in pigs. Ball R. Edmonton : University of Alberta, 134-136. International Symposium on Digestive Physiology in Pigs. 9, Banff, Canada, 14 Mai 2003/17 Mai 2003.

Résumé : A specific ELISA for pig gastrointestinal mucin was set up after mucin purification and specific antibody preparation. The aim was to quantify mucin contribution to ileal endogenous losses. This assay was sensitive, reproducible, and recognized well mucin in stomach and intestinal scrapings of pigs but not trout. Cross-reactivity was noted with digesta of chicken, rabbits and rats. lleal mucin flow was measured in weaned piglets fed diet based alone or mixed with chickpeas. It increased by 2.2 and 1.5-folds with white and black chickpeas, respectively. However, this did not reach significance due to high individual variation.

Mots-clés Agrovoc : porcelet, digestibilité, expérimentation, régime alimentaire

Classification Agris : L73 - Maladies des animaux
L50 - Physiologie et biochimie animales

Auteurs et affiliations

• Piel Christelle
• Montagne Lucile
• Salgado Paulo, CIRAD-EMVT-PPA (VNM)
• Lallès Jean-Paul, INRA (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/519795/)

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