Scopoletin expression in elicitor-treated and tobacco mosaic virus-infected tobacco plants

Costet Laurent, Fritig Bernard, Kauffmann Serge. 2002. Scopoletin expression in elicitor-treated and tobacco mosaic virus-infected tobacco plants. Physiologia Plantarum, 115 (2) : 228-235.

Résumé : Localized acquired resistance (LAR) characterizes a narrow zone of living cells expressing strong defense responses and surrounding cells undergoing a hypersensitive response (HR). In Samsun NN tobacco plants, tissues undergoing tobacco mosaic virus-induced or elicitor-induced LAR exhibit a strong blue fluorescence under UV light. 1% have shown that scopoletin and its glucoside, scopolin, accounted for the fluorescence: (1) both compounds were identified after extraction and purification by thin layer and high performance liquid chromatography; (2) there was a strict correlation between the occurrence of fluorescence and accumulation of high amounts of scopoletin; and (3) infiltration of commercial scopoletin caused a similar fluorescence to that occurring in LAR tissues. There was a 20-fold increase in scopoletin levels in LAR tissues compared to tissues treated with a non-HR dose of elicitor, while PR1 protein accumulated in similar amounts in both types of tissues. Scopoletin was able to suppress the elicitor-induced HR only when co-infiltrated with very low HR-dose of elicitor. These two observations suggested that, although scopoletin alone would not be able to control the development of the HR through its known antioxidant activity, it may nevertheless participate to such function of LAR tissues in combination with other antioxidant molecules.

Mots-clés Agrovoc : Nicotiana tabacum, réponse de la plante, mécanisme de défense, réponse immunitaire

Classification Agris : H20 - Maladies des plantes
F60 - Physiologie et biochimie végétale

Auteurs et affiliations

• Costet Laurent, CIRAD-CA-Canne à sucre (REU) ORCID: 0000-0003-3199-2885
• Fritig Bernard
• Kauffmann Serge, IBMP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/519801/)

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