A pharmocological approach to test the diffusible signal activity of reactive oxygen intermediates in elicitor-treated tobacco leaves

Costet Laurent, Dorey Stéphan, Fritig Bernard, Kauffmann Serge. 2002. A pharmocological approach to test the diffusible signal activity of reactive oxygen intermediates in elicitor-treated tobacco leaves. Plant and Cell Physiology, 43 (1) : 91-98.

Résumé : The capacity of H2O2, the most stable of the reactive oxygen species (ROI), to diffuse freely across biological membranes and to signal gene expression suggests that H202 could function as a short-lived second messenger diffusing from cell to cell. We tested this hypothesis in tobacco plants treated with a glycoprotein elicitor. Applied at 50 nM, it induces H2O2 accumulation and the hypersensitive response restricted to the infiltrated zone 1 tissue. Stimulation of a set of defense responses also occurs in the surrounding zone 2 tissue without diffusion of the elicitor. ROI levels in zone 1 were modulated using N-acetyl-L-cysteine (NAC) as a ROI scavenger and Rose Bengal (RB) as a ROI generator. We found that ROI appeared to act as signalling intermediates in pathways leading to salicylic acid accumulation, to PR1, PR5 and 3-hydroxy-3-methylglutarylCoA reductase expression in glycoprotein-treated zone 1 tissues. Compared to the treatment with the elicitor alone, coinfiltration of the glycoprotein and NAC increased the surface of zone 2 showing PR1 and O-methyltransferase expression. Application of RB had the opposite effect. The data suggest that, in our system, ROI did not act as a cellto-cell diffusible signal to activate PR protein and O-methyltransferase expression in zone 2.

Mots-clés Agrovoc : Nicotiana tabacum, réponse de la plante, réponse immunitaire, péroxyde d'hydrogène, oxydant

Classification Agris : F60 - Physiologie et biochimie végétale
H20 - Maladies des plantes

Auteurs et affiliations

• Costet Laurent, CIRAD-CA-Canne à sucre (REU) ORCID: 0000-0003-3199-2885
• Dorey Stéphan, John Innes Centre (GBR)
• Fritig Bernard
• Kauffmann Serge, IBMP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/519802/)

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