Folliot Michel, Galzi Serge, Laboureau Nathalie, Caruana Marie-Line, Teycheney Pierre-Yves, Côte François-Xavier.
2004. Risk assessing of spreading BSV through in vitro culture.
In : First International congress on Musa: harnessing research for improved livelihoods, 6-9 July 2004, Penang, Malaysia. Abstract guide. Picq Claudine (ed.), Vézina Anne (ed.). INIBAP, MARDI, BAPNET, University of Malaya, University of Putra, IPGRI
Résumé : #In vitro# multiplication is often reported as the main cause for triggering the production of episomal infectious BSV particles in banana hybrid species harbouring some or all of the B (#Musa balbisiana#) genome, through the activation of BSV endogenous pararetrovirus (EPRV) sequences integrated into the B genome. Nevertheless, mass production of vitroplantlets remains the most widely used method for the diffusion of either #Musa# germplasm or new improved hybrid species. A better understanding of the effects of in vitro culture on BSV EPRV activation is thus necessary to evaluate the risks of spreading BSV through the wide diffusion of banana plantlets. In order to evaluate this risk, CIRAD and INIBAP are developing a collaborative project aimed at answering the following questions: Does BSV EPRV activation occur through in vitro culture in all inter-specific hybrid species and in " natural " plantain species too? Is there a correlation between the duration of #in vitro# subculture steps and the percentage of plantlets exhibiting BSV episomal particles ? In order to answer these questions, three different accessions were used: two natural triploid plantains (AAB) - 'Kelong Mekintu' (KM) and 'Black Penkelon' (PK)- and the tetraploid hybrid (AAAB) - CRBP 39. Virus-free suckers were selected and mass propagated using standard in vitro budding methods. During the successive multiplication (proliferation) subcultures, at least 40 shoots were randomly picked and screened for the presence of episomal BSV particles, using standard immuno-capture PCR based detection methods. BSV episomal particles were detected during in vitro culture in both natural plantains and CRBP39 hybrid, with BSV-Ol being the predominantly detected BSV strain . Both natural plantains and CRBP39 displayed similar patterns of activation. Percentages of plantlets indexed positive for BSV-Ol rapidly increased after the first subculture cycles. Depending on cultivars, maximum percentages of BSV-Ol positive plantlets ranged between 10 % (cv. 'Penkelon' and 'CRBP 39') and 20 % (cv. 'Kelong Mekintu') and were reached for TPS (total produced shoots) values between 800 and 2000. Following this increase step, a steady state phase was observed. Then the percentage of BSV-Ol positive plantlets decreased for the three cultivars studied when increasing the number of subcultures. This was especially striking for CRBP 39 hybrid, for which values of zero (i.e below the sensitivity threshold of detection tests) were reached from TPS values of 4000 onwards although the decrease was less pronounced for the two other cultivars studied. These results will be presented and their outcome for in vitro mass propagation and diffusion of #Musa# germplasm will be discussed (Texte intégral).
Mots-clés Agrovoc : Musa, ressource génétique, virus des végétaux, culture in vitro, multiplication des plantes, Musa balbisiana
Classification Agris : H20 - Maladies des plantes
F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Folliot Michel, CIRAD-FLHOR-BPA (MTQ)
- Galzi Serge, CIRAD-FLHOR-BPA (FRA)
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Laboureau Nathalie, CIRAD-FLHOR-BPA (FRA)
ORCID: 0000-0002-0105-3919
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Caruana Marie-Line, CIRAD-AMIS-PROTECTION DES CULTURES (FRA) ORCID: 0000-0003-4486-2449
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Teycheney Pierre-Yves, CIRAD-FLHOR-BPA (GLP) ORCID: 0000-0002-9754-0745
- Côte François-Xavier, CIRAD-FLHOR-BPA (FRA)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/520074/)
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