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Isozymes and DNA restriction fragment length polymorphisms (RFLP'S) as genetic markers in citrus selection

Ollitrault Patrick. 1990. Isozymes and DNA restriction fragment length polymorphisms (RFLP'S) as genetic markers in citrus selection. In : Rehabilitation of citrus industry in the Asia Pacific region : proceedings of the Asia Pacific international conference on citriculture, Chiang Mai, Thailand, 4-10th February 1990. Aubert Bernard (ed.), Tontyaporn Sanchai (ed.), Buangsuwon D. (ed.). FAO. Rome : FAO, 57-68. International FAO-UNDP Conference on Rehabilitation of Citrus Industry in the Asia Pacific Region. 4, Chiang Mai, Thaïlande, 4 Février 1990/10 Février 1990.

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Résumé : Isozymes and restriction fragment length polymorphisms (RFLPs) are convenient genetic markers for plant breeders and plant taxonomists. The application of these molecular markers to citrus genetics is discussed here. Isozymes have proven reliable tools for distinguishing nucellar from zygotic plants and monitoring in vitro, culture tissues. They are also helpful for assessing the citrus genetic organization: cultivar identification, heterozygosity, phylogeny. RFLP's have a similar scope of application, but can secure a greater resolving power. As a reason, they are used for exploring the entire genome, or for constructing detailed genetic mapping where the genes with desirable traits will be localized.

Classification Agris : F30 - Génétique et amélioration des plantes

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