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Genotyping of mature trees of Entandrophragma cylindricum with microsatellites

Genotyping of mature trees of Entandrophragma cylindricum with microsatellites. Garcia Fabien, Noyer Jean-Louis, Risterucci Ange-Marie, Chevallier Marie-Hélène. 2004. Journal of Heredity, 95 (5) : 454-457.
http://dx.doi.org/10.1093/jhered/esh071

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Article de revue ; Article de revue à facteur d'impact

Résumé : We have characterized 10 microsatellite loci for the tropical tree Entandrophragma cylindricum (Sprague) Sprague (sapelli) in order to genotype individuals in forest stands for estimation of the genetic diversity of the species. We used the technique of building a (GA)n microsatellite¿enriched library by capture with streptavidin-coated magnetic beads. We assessed the polymorphism of seven microsatellites in 186 mature trees in a selectively logged stand (Dimako) and an unlogged stand (Ndama), both in Cameroon. All the loci were polymorphic, and the number of alleles was high, ranging from eight to 36, with a mean of 22.1. Both stands showed the same high level of genetic diversity (mean HE = 0.85) and a low genetic differentiation (Fst = 0.007), indicating that genetic diversity was within rather than among populations. Five and three out seven loci in Dimako and Ndama, respectively, showed a deficit of heterozygotes. The seven loci enabled more than 97% of the mature trees in each stand to be identified. It was concluded that these markers can be efficiently used for gene flow studies. (Résumé d'auteur)

Mots-clés Agrovoc : Meliaceae, Variation génétique, Génotype, Identification, Microsatellite, Marqueur génétique

Mots-clés géographiques Agrovoc : Cameroun

Mots-clés complémentaires : Entandrophragma cylindricum

Classification Agris : F30 - Génétique et amélioration des plantes

Document en bibliothèque : http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=182774

Localisation du document : CD_BR12627 [(Bibliothèque de Lavalette)] ; BA_BR2877 [(Bibliothèque de Baillarguet)]

Auteurs et affiliations

  • Garcia Fabien
  • Noyer Jean-Louis, CIRAD-AMIS-BIOTROP (FRA)
  • Risterucci Ange-Marie, CIRAD-AMIS-BIOTROP (FRA)
  • Chevallier Marie-Hélène, CIRAD-FORET-FORETS NATURELLES (FRA)

Source : Cirad - Agritrop

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