Purification and characterization of two iron superoxide dismutases of Phytomonas sp. isolated from Euphorbia characias (plant trypanosomatids)

Marin Clotilde, Rodriguez-Gonzalez Isabel, Hitos Ana B., Rosales M.J., Dollet Michel, Sanchez Moreno Manuel. 2004. Purification and characterization of two iron superoxide dismutases of Phytomonas sp. isolated from Euphorbia characias (plant trypanosomatids). Parasitology, 129 (1) : 79-86.

Autre titre : Purification et caractérisation de deux superoxyde dismutases ferriques de Phytomonas sp. isolé sur Euphorbia characias (trypanosomes des plantes)

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Résumé : Two superoxide dismutases (SODI and SODII) have been purified by differential centrifugation, fractionation with ammonium sulphate followed by chromatographic separation (ionic exchange and affinity), from a plant trypanosomatid isolated from #Euphorbia characias#, and then characterized for several biochemical properties. Both enzymes were insensitive to cyanide but sensitive to hydrogen peroxide, properties characteristic of iron-containing superoxide dismutase. SODI had a molecular mass of approximately 66 kDa, whereas the molecular mass of SODII was approximately 22 kDa, both enzymes showing single bands. The isoelectric points of SODI and SODII were 6.8 and 3.6, respectively. The enzymatic stability persisted at least for 6 months when the sample was lyophilized and preserved at - 80°C. Digitonin titration and subcellular fractionation showed that both enzymes were in the cytoplasmic fraction, although part of SODII isoenzyme was also associated with glycosomes. We assayed these activities (SOD) in 18 trypanosomatid isolates on isoelectric focusing gels, and have demonstrated that the SOD is a biochemical marker sufficient to identify a trypanosomatid isolated from a plant as belonging to the genus #Phytomonas# and to distinguish between a true #Phytomonas# and other trypanosomatids that are capable of causing transient infections in plants.

Mots-clés Agrovoc : Phytomonas, Mastigophora, Euphorbia characias, superoxyde dismutase, technique analytique, marqueur génétique

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Marin Clotilde, Instituto de Biotecnologia (ESP)
• Rodriguez-Gonzalez Isabel, Instituto de Biotecnologia (ESP)
• Hitos Ana B., Instituto de Biotecnologia (ESP)
• Rosales M.J., Instituto de Biotecnologia (ESP)
• Dollet Michel, CIRAD-EMVT-SANTE ANIMALE (FRA)
• Sanchez Moreno Manuel, Instituto de Biotecnologia (ESP)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/523130/)

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