Isozyme analysis revealed that the Portuguese mandarin Carvalhais originated as a single clone

Elisiario Paulo J., Santos Gilberto G., Guerreiro Antonio R., Ollitrault Patrick, Luro François, Leitão José Manuel. 1999. Isozyme analysis revealed that the Portuguese mandarin Carvalhais originated as a single clone. Scientia Horticulturae, 82 (1-2) : 145-152.

Résumé : 'Carvalhais' mandarin is a well-known Portuguese mandarin used in several Citrus breeding programmes throughout the world. The first step in the implementation of a breeding programme of this variety was to screen for its natural genetic variability. A significant number of adult trees and young seedlings were submitted to isozyme analysis. For better interpretation of the isozyme patterns of 'Carvalhais', several plants resulting from crosses between this Portuguese mandarin cultivar and the monoembryonic mandarin cultivar Clementine MA3 SRA 649, were also analysed. All the analysed trees of 'Carvalhais' showed similar monomorphic patterns for each isozyme system, indicating that this mandarin cultivar originated as a single initial clone. Young seedlings exhibited monomorphic isozyme patterns identical to the adult plants revealing a high level of apomixis in this cultivar.

Mots-clés Agrovoc : Citrus reticulata, isoenzyme, marqueur génétique, amélioration des plantes, variation génétique, apomixie, culture in vitro

Mots-clés complémentaires : Variabilité génétique

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Elisiario Paulo J., Universidade do Algarve (PRT)
• Santos Gilberto G., Universidade do Algarve (PRT)
• Guerreiro Antonio R., Direcçao Regional de Agricultura do Algarve (PRT)
• Ollitrault Patrick, CIRAD-FLHOR-ARF (GLP) ORCID: 0000-0002-9456-5517
• Luro François, INRA (FRA)
• Leitão José Manuel, Universidade do Algarve (PRT)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/524199/)

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