Partial sequencing of the hrpB and endoglucanase genes confirms and expands the known diversity within the Ralstonia solanacearum species complex

Poussier Stéphane, Prior Philippe, Luisetti Jacques, Fegan Max. 2000. Partial sequencing of the hrpB and endoglucanase genes confirms and expands the known diversity within the Ralstonia solanacearum species complex. Systematic and Applied Microbiology, 23 : 479-486.

Résumé : We determined partial hrpB and endoglucanase genes sequences for 30 strains of Ralstonia solanacearum and one strain of the blood disease bacterium (BDB), a close relative of Ralstonia solanacearum. Sequence comparisons showed high levels of variability within these two regions of the genome involved in pathogenicity. Phytogenetic analysis based upon sequence comparisons of the two regions revealed three major clusters comprising all Rastonia solanacearum isolates, the BDB strain constituted a phyllogenetically distinct entity. Cluster 1 and cluster 2 correspondee to the previously defined divisions 1 and 2 of Ralstonia solanacearum. Moreover, two subclusters could be identified within cluster 2. The last cluster, designated cluster 3 in this study, included biovar 1 and N2 strains originating from Africa. This recently described group of strains was confirmed to clearly different from the other strains suggesting a separate evolution from those of both divisions 1 and 2. [résumé auteur]

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

• Poussier Stéphane, CIRAD-FLHOR-PRH (REU)
• Prior Philippe
• Luisetti Jacques, CIRAD-FLHOR-PRH (REU)
• Fegan Max

Source : Cirad - Agritrop (https://agritrop.cirad.fr/524235/)

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