Microsatellite-based high density linkage map in oil palm (Elaeis guineensis Jacq.)

Billotte Norbert, Marseillac Nicolas, Risterucci Ange-Marie, Adon Benjamin, Brottier Philippe, Baurens Franc-Christophe, Singh Rajinder, Herran Ana, Asmady H., Billot Claire, Amblard Philippe, Durand-Gasselin Tristan, Courtois Brigitte, Asmono D., Cheah Suan-Choo, Rohde Wolfgang, Ritter Enrique, Charrier André. 2005. Microsatellite-based high density linkage map in oil palm (Elaeis guineensis Jacq.). Theoretical and Applied Genetics, 110 (4) : 754-765.

Résumé : A microsatellite-based high-density linkage map for oil palm (Elaeis guinensis Jacq.) was constructed from a cross between two heterozygous parents, a tenera palm from the La Mé population (LM2T) and a dura palm from the Deli population (DA10D). A set of 390 simple sequence repeat (SSR) markers was developed in oil palm from microsatellite-enriched libraries and evaluated for polymorphism along with 21 coconut SSRs. A dense and genome-wide microsatellite framework as well as saturating amplified fragments length polymorphisms (AFLPs) allowed the construction of a linkage map consisting of 255 microsatellites, 688 AFLPs and the locus of the Sh gene, which controls the presence or absence of a shell in the oil palm fruit. An AFLP marker E-Agg/M-CAA132 was mapped at 4.7 cM from the Sh locus. The 944 genetic markers were distributed on 16 linkage groups (LGs) and covered 1,743 cM. Our linkage map is the first in oil palm to have 16 independent linkage groups corresponding to the plant's 16 homologous chromo-some pairs. It is also the only high-density linkage map with as many microsatellite markers in an Arecaceae species and represents an important step towards quantitative trait loci analysis and physical mapping in the E. guineensis species.

Mots-clés Agrovoc : Elaeis guineensis, huile de palme, microsatellite, marqueur génétique, carte génétique, PCR, polymorphisme génétique, Arecaceae, liaison génétique

Mots-clés complémentaires : AFLP

Classification Agris : F30 - Génétique et amélioration des plantes

Champ stratégique Cirad : Axe 1 (2005-2013) - Intensification écologique

Auteurs et affiliations

• Billotte Norbert, CIRAD-CP-PALMIER (FRA)
• Marseillac Nicolas, CIRAD-AMIS-UMR PIA (FRA)
• Risterucci Ange-Marie, CIRAD-AMIS-BIOTROP (FRA)
• Adon Benjamin, CNRA (CIV)
• Brottier Philippe, Centre national de séquençage (FRA)
• Baurens Franc-Christophe, CIRAD-AMIS-BIOTROP (FRA) ORCID: 0000-0002-5219-8771
• Singh Rajinder, MPOB (MYS)
• Herran Ana, Centro de Arkaute (ESP)
• Asmady H., SOCFINDO (IDN)
• Billot Claire, CIRAD-CP-PALMIER (FRA)
• Amblard Philippe, CIRAD-CP-PALMIER (FRA)
• Durand-Gasselin Tristan, CIRAD-CP-PALMIER (FRA)
• Courtois Brigitte, CIRAD-AMIS-BIOTROP (FRA) ORCID: 0000-0003-2118-7102
• Asmono D., IOPRI (IDN)
• Cheah Suan-Choo, MPOB (MYS)
• Rohde Wolfgang, MPIZ (DEU)
• Ritter Enrique, Centro de Arkaute (ESP)
• Charrier André, ENSAM [Ecole nationale supérieure agronomique de Montpellier] (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/524912/)

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