Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial

Bashiruddin John B., Frey Joachim, Heldtander Königsson Malin, Johansson Karl-Erik, Hotzel Helmut, Diller Roland, De Santis Paola, Botelho Ana, Ayling Roger D., Nicholas Robin A.J., Thiaucourt François, Sachse Konrad. 2005. Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: a collaborative trial. Veterinary Journal, 169 (2) : 268-275.

Résumé : Diagnostic differentiation between the ruminant pathogens Mycoplasma agalactiae and Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for M. agalactiae and three systems for M. bovis on a total of 41 strains of the two Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three M. bovis strains from small ruminants and two M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes.

Mots-clés Agrovoc : Mycoplasma bovis, Mycoplasma, biochimie, identification, Mycoplasma agalactiae

Classification Agris : L50 - Physiologie et biochimie animales

Champ stratégique Cirad : Axe 4 (2005-2013) - Santé animale et maladies émergentes

Auteurs et affiliations

• Bashiruddin John B.
• Frey Joachim, Institut de bactériologie vétérinaire (CHE)
• Heldtander Königsson Malin, National Veterinary Institute (SWE)
• Johansson Karl-Erik, National Veterinary Institute (SWE)
• Hotzel Helmut, Institut für Bakterielle Infektionen und Zoonosen (DEU)
• Diller Roland, Institut für Bakterielle Infektionen und Zoonosen (DEU)
• De Santis Paola, IZSA&M (ITA)
• Botelho Ana, Ministério da Agricultura do Desenvolvimento Rural e das Pescas (Portugal) (PRT)
• Ayling Roger D., Veterinary Laboratories Agency (GBR)
• Nicholas Robin A.J., Veterinary Laboratories Agency (GBR)
• Thiaucourt François, CIRAD-EMVT-SANTE ANIMALE (FRA)
• Sachse Konrad, Institut für Bakterielle Infektionen und Zoonosen (DEU)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/525758/)

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