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Molecular markers to assess genetic diversity and structure of Pterocarpus officinalis Jack and its associated micro-organisms in the Marshy forests

Muller Félix, Bouvet Jean-Marc, Béna Gilles, Vaillant Alexandre, Prin Yves, Le Roux Christine, Rousteau Alain, Dreyfus Bernard, Bâ Amadou Moustapha. 2005. Molecular markers to assess genetic diversity and structure of Pterocarpus officinalis Jack and its associated micro-organisms in the Marshy forests. In : Abstracts of Plant and Animal Genomes XIIIth Conference, San Diego, CA (USA), January 15-19, 2005. s.l. : s.n., Résumé Plant and Animal Genomes Conference. 13, San Diego, États-Unis, 15 January 2005/19 January 2005.

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Abstract : The influences of the combined swamp environment and geographical structure within the natural range of tree/rhizobia symbiotic systems are poorly documented. Pterocarpus officinalis was selected as an interesting species to analyze the genetic structuring of two symbiotic partners in a continental and insular system. We sampled 7 P.officinalis populations in different West Indies islands (Guadeloupe, Dominica, Martinique, Puerto Rico) and in French Guiana on the continent. Between 20 and 30 individuals were sampled in each population and for each individual, 2 leaves and 5 nodules were collected for future molecular analyses. Genetic diversity of P.officinalis was assessed through nuclear and cloroplastic microsatellite. The genetic diversity of symbiotic bacteria isolated from P.officinalis nodules was investigated by sequence analysis of 16S-23S rDNA intergenic spacer (IGS). Bacterial diversity results showed a very low genetic diversity in insular system and a high diversity on the continent. Ours first results showed for the plants a strong population structure, with a low genetic diversity in insular system and a high diversity on the continent. Chloroplastic microsatellite analyses showed however a limited genetic structure (compared to other tree species) which could be explained by the water floating mode of seeds dispersal. Nuclear microsatellite analyses showed complex pattern of correlation between geographic and genetic distance. These results suggest a parallel pattern in molecular diversity for the plants and their bacteria, possible consequence of similar impact of evolutionary forces (limited gene flow and strong genetic drift). (Texte intégral)

Mots-clés Agrovoc : Pterocarpus, Marqueur génétique, Symbiose, Bacteria, Génétique moléculaire, Provenance

Classification Agris : K10 - Forestry production
F30 - Plant genetics and breeding

Auteurs et affiliations

  • Muller Félix, CIRAD-FORET-UPR Génétique forestière (FRA)
  • Bouvet Jean-Marc, CIRAD-FORET-UPR Génétique forestière (FRA)
  • Béna Gilles, CIRAD-AMIS-UMR LSTM (FRA)
  • Vaillant Alexandre, CIRAD-FORET-UPR Génétique forestière (FRA) ORCID: 0000-0002-4785-1350
  • Prin Yves, CIRAD-AMIS-UMR LSTM (FRA) ORCID: 0000-0002-3706-0045
  • Le Roux Christine, CIRAD-AMIS-UMR LSTM (FRA) ORCID: 0000-0003-0668-3589
  • Rousteau Alain, UAG (GLP)
  • Dreyfus Bernard, IRD (FRA)
  • Bâ Amadou Moustapha, UAG (GLP)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/525794/)

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