Etienne Hervé, Solano William, Pereira A., Bertrand Benoît, Vasquez Nelly, Barry-Etienne Dominique.
2000. A solution for the utilization of in vitro culture for mass-diffusion of Coffea arabica elite materials : The direct sowing of somatic embryos produced in a bioreactor.
In : XIX Simposio Latinoamericano de caficultura del 2 al 6 de octubre, San José, Costa Rica. Memoria. IICA-PROMECAFE, ICAFE
Résumé : The effect of germination conditions on the morphology of Coffea arabica L. somatic embryos mass-produced in a one-litre temporary immersion bioreactor (RITA7) was studied with emphasis on direct sowing in soil. Using germinated embryos, direct sowing resulted in a highly successful conversion of embryos into plants. Culture density above 1,600 embryos/11-bioreactor positively affected embryo morphology by causing higher embryonic axis elongation (+4-5mm). At this density, addition of a high sucrose concentration (234 mM), 2 weeks before sowing, promoted an increase in effective plant conversion in soil (78%) and a vigorous vegetative growth of the resulting plants. Using this somatic embryogenesis process, 50 000 selected F1 hybrids were recently difunded in America Central.
Classification Agris : F02 - Multiplication végétative des plantes
Auteurs et affiliations
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Etienne Hervé, CIRAD-CP-CAFE (CRI)
ORCID: 0000-0002-6208-9982
- Solano William, IICA (CRI)
- Pereira A., IICA (CRI)
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Bertrand Benoît, CIRAD-CP-CAFE (CRI) ORCID: 0000-0003-1969-3479
- Vasquez Nelly, CATIE (CRI)
- Barry-Etienne Dominique, CATIE (CRI)
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/526175/)
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